From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:2c44.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:2c44.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_2c44| PDB=2c44 | SCENE= }} | | {{STRUCTURE_2c44| PDB=2c44 | SCENE= }} |
| | | | |
| - | '''CRYSTAL STRUCTURE OF E. COLI TRYPTOPHANASE'''
| + | ===CRYSTAL STRUCTURE OF E. COLI TRYPTOPHANASE=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | Pyridoxal 5'-phosphate (PLP) dependent tryptophanase has been isolated from Escherichia coli and its crystal structure has been determined. The structure shares the same fold with and has similar quaternary structure to Proteus vulgaris tryptophanase and tyrosine-phenol lyase, but is found in a closed conformation when compared with these two enzymes. The tryptophanase structure, solved in its apo form, does not have covalent PLP bound in the active site, but two sulfate ions. The sulfate ions occupy the phosphoryl-binding site of PLP and the binding site of the alpha-carboxyl of the natural substrate tryptophan. One of the sulfate ions makes extensive interactions with both the transferase and PLP-binding domains of the protein and appears to be responsible for holding the enzyme in its closed conformation. Based on the sulfate density and the structure of the P. vulgaris enzyme, PLP and the substrate tryptophan were modeled into the active site. The resulting model is consistent with the roles of Arg419 in orienting the substrate to PLP and acidifying the alpha-proton of the substrate for beta-elimination, Lys269 in the formation and decomposition of the PLP quinonoid intermediate, Arg230 in orienting the substrate-PLP intermediates in the optimal conformation for catalysis, and His463 and Tyr74 in determining substrate specificity and suggests that the closed conformation observed in the structure could be induced by substrate binding and that significant conformational changes occur during catalysis. A catalytic mechanism for tryptophanase is proposed. Since E. coli tryptophanase has resisted forming diffraction-quality crystals for many years, the molecular surface of tryptophanase has been analyzed in various crystal forms and it was rationalized that strong crystal contacts occur on the flat surface of the protein and that the size of crystal contact surface seems to correlate with the diffraction quality of the crystal.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16790938}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16790938 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_16790938}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 29: |
Line 33: |
| | [[Category: Pyridoxal phosphate]] | | [[Category: Pyridoxal phosphate]] |
| | [[Category: Tryptophan catabolism]] | | [[Category: Tryptophan catabolism]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:13:20 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:45:05 2008'' |
Revision as of 05:45, 28 July 2008
Template:STRUCTURE 2c44
CRYSTAL STRUCTURE OF E. COLI TRYPTOPHANASE
Template:ABSTRACT PUBMED 16790938
About this Structure
2C44 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of Escherichia coli tryptophanase., Ku SY, Yip P, Howell PL, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):814-23. Epub 2006, Jun 20. PMID:16790938
Page seeded by OCA on Mon Jul 28 08:45:05 2008
