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1w1o

From Proteopedia

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[[Image:1w1o.gif|left|200px]]
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{{Seed}}
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[[Image:1w1o.png|left|200px]]
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{{STRUCTURE_1w1o| PDB=1w1o | SCENE= }}
{{STRUCTURE_1w1o| PDB=1w1o | SCENE= }}
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'''NATIVE CYTOKININ DEHYDROGENASE'''
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===NATIVE CYTOKININ DEHYDROGENASE===
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==Overview==
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Cytokinins form a diverse class of compounds that are essential for plant growth. Cytokinin dehydrogenase has a major role in the control of the levels of these plant hormones by catalysing their irreversible oxidation. The crystal structure of Zea mays cytokinin dehydrogenase displays the same two-domain topology of the flavoenzymes of the vanillyl-alcohol oxidase family but its active site cannot be related to that of any other family member. The X-ray analysis reveals a bipartite architecture of the catalytic centre, which consists of a funnel-shaped region on the protein surface and an internal cavity lined by the flavin ring. A pore with diameter of about 4A connects the two active-site regions. Snapshots of two critical steps along the reaction cycle were obtained through the structural analysis of the complexes with a slowly reacting substrate and the reaction product, which correspond to the states immediately before (Michaelis complex) and after (product complex) oxidation has taken place. The substrate displays a "plug-into-socket" binding mode that seals the catalytic site and precisely positions the carbon atom undergoing oxidation in close contact with the reactive locus of the flavin. A polarising H-bond between the substrate amine group and an Asp-Glu pair may facilitate oxidation. Substrate to product conversion results in small atomic movements, which lead to a planar conformation of the reaction product allowing double-bond conjugation. These features in the mechanism of amine recognition and oxidation differ from those observed in other flavin-dependent amine oxidases.
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(as it appears on PubMed at http://www.pubmed.gov), where 15321719 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15321719}}
==About this Structure==
==About this Structure==
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[[Category: Flavoprotein]]
[[Category: Flavoprotein]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:01:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:55:39 2008''

Revision as of 05:55, 28 July 2008

Image:1w1o.png

Template:STRUCTURE 1w1o

NATIVE CYTOKININ DEHYDROGENASE

Template:ABSTRACT PUBMED 15321719

About this Structure

1W1O is a Single protein structure of sequence from Zea mays. Full crystallographic information is available from OCA.

Reference

Structures of Michaelis and product complexes of plant cytokinin dehydrogenase: implications for flavoenzyme catalysis., Malito E, Coda A, Bilyeu KD, Fraaije MW, Mattevi A, J Mol Biol. 2004 Aug 27;341(5):1237-49. PMID:15321719

Page seeded by OCA on Mon Jul 28 08:55:39 2008

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