This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3brg

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:3brg.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:3brg.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_3brg| PDB=3brg | SCENE= }}
{{STRUCTURE_3brg| PDB=3brg | SCENE= }}
-
'''CSL (RBP-Jk) bound to DNA'''
+
===CSL (RBP-Jk) bound to DNA===
-
==Overview==
+
<!--
-
The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.
+
The line below this paragraph, {{ABSTRACT_PUBMED_18381292}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 18381292 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_18381292}}
==About this Structure==
==About this Structure==
Line 36: Line 40:
[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:33:37 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 08:59:23 2008''

Revision as of 05:59, 28 July 2008

Image:3brg.png

Template:STRUCTURE 3brg

CSL (RBP-Jk) bound to DNA

Template:ABSTRACT PUBMED 18381292

About this Structure

3BRG is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:18381292

Page seeded by OCA on Mon Jul 28 08:59:23 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3brg"
Personal tools