From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:2i21.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:2i21.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_2i21| PDB=2i21 | SCENE= }} | | {{STRUCTURE_2i21| PDB=2i21 | SCENE= }} |
| | | | |
| - | '''Bacteriorhodopsin/lipid complex, T46V mutant'''
| + | ===Bacteriorhodopsin/lipid complex, T46V mutant=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The X-ray diffraction structure of the non-illuminated D96A bacteriorhodopsin mutant reveals structural changes as far away as 15 A from residue 96, at the retinal, Trp-182, Ala-215, and waters 501, 402, and 401. The Asp-to-Ala side-chain replacement breaks its hydrogen bond with Thr-46, and the resulting separation of the cytoplasmic ends of helices B and C is communicated to the retinal region through a chain of covalent and hydrogen bonds. The unexpected long-range consequences of the D96A mutation include breaking the hydrogen bond between O of Ala-215 and water 501 and the formation of a new hydrogen bond between water molecules 401 and 402 in the extracellular region. Because in the T46V mutant a new water molecule appears at Asp-96 and its hydrogen-bond to Ile-45 replaces Thr-46 as its link to helix B, the separation of helices B and C is smaller than that in D96A, and there are no atomic displacements elsewhere in the protein. Propagation of conformational changes along the chain between the retinal and Thr-46 had been observed earlier in the crystal structures of the D96N and E204Q mutants but in the trapped M state. Consistent with the perturbation of the retinal region in D96A, little change of the Thr-46 region occurs between the non-illuminated and M states of this mutant. It appears that a local perturbation can propagate along a track in both directions between the retinal and the Asp-96/Thr-46 pair, either from photoisomerization of the retinal in the wild-type protein in one case or from the D96A mutation in the other. | + | The line below this paragraph, {{ABSTRACT_PUBMED_17002299}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17002299 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_17002299}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 33: |
Line 37: |
| | [[Category: Retinal protein]] | | [[Category: Retinal protein]] |
| | [[Category: Serpentine]] | | [[Category: Serpentine]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:59:04 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:02:42 2008'' |
Revision as of 06:02, 28 July 2008
Template:STRUCTURE 2i21
Bacteriorhodopsin/lipid complex, T46V mutant
Template:ABSTRACT PUBMED 17002299
About this Structure
2I21 is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.
Reference
Propagating structural perturbation inside bacteriorhodopsin: crystal structures of the M state and the D96A and T46V mutants., Lanyi JK, Schobert B, Biochemistry. 2006 Oct 3;45(39):12003-10. PMID:17002299
Page seeded by OCA on Mon Jul 28 09:02:42 2008
