1ree

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[[Image:1ree.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1ree| PDB=1ree | SCENE= }}
{{STRUCTURE_1ree| PDB=1ree | SCENE= }}
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'''ENDO-1,4-BETA-XYLANASE II COMPLEX WITH 3,4-EPOXYBUTYL-BETA-D-XYLOSIDE'''
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===ENDO-1,4-BETA-XYLANASE II COMPLEX WITH 3,4-EPOXYBUTYL-BETA-D-XYLOSIDE===
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==Overview==
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The three-dimensional structures of endo-1,4-xylanase II (XYNII) from Trichoderma reesei complexed with 4,5-epoxypentyl beta-D-xyloside (X-O-C5),3,4-epoxybutyl beta-D-xyloside (X-O-C4), and 2,3-epoxypropyl beta-D-xyloside (X-O-C3) were determined by X-ray crystallography. High-resolution measurement revealed clear electron densities for each ligand. Both X-O-C5 and X-O-C3 were found to form a covalent bond with the putative nucleophile Glu86. Unexpectedly, X-O-C4 was found to bind to the putative acid/base catalyst Glu177. In all three complexes, clear conformational changes were found in XYNII compared to the native structure. These changes were largest in the X-O-C3 complex structure.
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(as it appears on PubMed at http://www.pubmed.gov), where 8755744 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8755744}}
==About this Structure==
==About this Structure==
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[[Category: Xylan degradation]]
[[Category: Xylan degradation]]
[[Category: Xylanase]]
[[Category: Xylanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:23:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:03:28 2008''

Revision as of 06:03, 28 July 2008

Image:1ree.png

Template:STRUCTURE 1ree

ENDO-1,4-BETA-XYLANASE II COMPLEX WITH 3,4-EPOXYBUTYL-BETA-D-XYLOSIDE

Template:ABSTRACT PUBMED 8755744

About this Structure

1REE is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

Reference

Covalent binding of three epoxyalkyl xylosides to the active site of endo-1,4-xylanase II from Trichoderma reesei., Havukainen R, Torronen A, Laitinen T, Rouvinen J, Biochemistry. 1996 Jul 23;35(29):9617-24. PMID:8755744

Page seeded by OCA on Mon Jul 28 09:03:28 2008

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