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| | {{STRUCTURE_2qb7| PDB=2qb7 | SCENE= }} | | {{STRUCTURE_2qb7| PDB=2qb7 | SCENE= }} |
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| - | '''Saccharomyces cerevisiae cytosolic exopolyphosphatase, phosphate complex'''
| + | ===Saccharomyces cerevisiae cytosolic exopolyphosphatase, phosphate complex=== |
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| - | ==Overview==
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| - | Inorganic long-chain polyphosphate is a ubiquitous linear polymer in biology, consisting of many phosphate moieties linked by phosphoanhydride bonds. It is synthesized by polyphosphate kinase, and metabolised by a number of enzymes, including exo- and endopolyphosphatases. The Saccharomyces cerevisiae gene PPX1 encodes for a 45 kDa, metal-dependent, cytosolic exopolyphosphatase that processively cleaves the terminal phosphate group from the polyphosphate chain, until inorganic pyrophosphate is all that remains. PPX1 belongs to the DHH family of phosphoesterases, which includes: family-2 inorganic pyrophosphatases, found in Gram-positive bacteria; prune, a cyclic AMPase; and RecJ, a single-stranded DNA exonuclease. We describe the high-resolution X-ray structures of yeast PPX1, solved using the multiple isomorphous replacement with anomalous scattering (MIRAS) technique, and its complexes with phosphate (1.6 A), sulphate (1.8 A) and ATP (1.9 A). Yeast PPX1 folds into two domains, and the structures reveal a strong similarity to the family-2 inorganic pyrophosphatases, particularly in the active-site region. A large, extended channel formed at the interface of the N and C-terminal domains is lined with positively charged amino acids and represents a conduit for polyphosphate and the site of phosphate hydrolysis. Structural comparisons with the inorganic pyrophosphatases and analysis of the ligand-bound complexes lead us to propose a hydrolysis mechanism. Finally, we discuss a structural basis for substrate selectivity and processivity.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_17599355}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 17599355 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_17599355}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Dhh family phosphatase]] | | [[Category: Dhh family phosphatase]] |
| | [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 14:39:48 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:09:08 2008'' |
Revision as of 06:09, 28 July 2008
Template:STRUCTURE 2qb7
Saccharomyces cerevisiae cytosolic exopolyphosphatase, phosphate complex
Template:ABSTRACT PUBMED 17599355
About this Structure
2QB7 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The crystal structure of the cytosolic exopolyphosphatase from Saccharomyces cerevisiae reveals the basis for substrate specificity., Ugochukwu E, Lovering AL, Mather OC, Young TW, White SA, J Mol Biol. 2007 Aug 24;371(4):1007-21. Epub 2007 May 31. PMID:17599355
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