1m5w
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(New page: 200px<br /><applet load="1m5w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m5w, resolution 1.96Å" /> '''1.96 A Crystal Struc...)
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Revision as of 19:02, 20 November 2007
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1.96 A Crystal Structure of Pyridoxine 5'-Phosphate Synthase in Complex with 1-deoxy-D-xylulose phosphate
Overview
We report the 1.96 A crystal structure of pyridoxine 5'-phosphate synthase, (PdxJ) in complex with 1-deoxy-D-xylulose phosphate (dXP). The octameric, enzyme possesses eight distinct binding sites, and three different binding, states are observed. The observation of these three states supports a, mechanism in which precise conformational changes of a peptide loop and, groups of active site residues modulate binding and specificity. The, differences in protein conformation when one or two substrates are bound, can be correlated with a condensation mechanism that leads productively to, the formation of pyridoxine 5'-phosphate (PNP). "Snapshots" of the, progression from the apo form to a singly occupied "transitional binding", state and, subsequently, to a fully occupied, reactive state are revealed, and indicate how the enzyme structure can be related to a plausible, catalytic mechanism and, moreover, to favorable energetics of reaction.
About this Structure
1M5W is a Single protein structure of sequence from Escherichia coli with DXP and PO4 as ligands. Full crystallographic information is available from OCA.
Reference
Multistate binding in pyridoxine 5'-phosphate synthase: 1.96 A crystal structure in complex with 1-deoxy-D-xylulose phosphate., Yeh JI, Du S, Pohl E, Cane DE, Biochemistry. 2002 Oct 1;41(39):11649-57. PMID:12269807
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