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1uwm

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[[Image:1uwm.gif|left|200px]]
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{{STRUCTURE_1uwm| PDB=1uwm | SCENE= }}
{{STRUCTURE_1uwm| PDB=1uwm | SCENE= }}
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'''REDUCED FERREDOXIN 6 FROM RHODOBACTER CAPSULATUS'''
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===REDUCED FERREDOXIN 6 FROM RHODOBACTER CAPSULATUS===
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==Overview==
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FdVI from Rhodobacter capsulatus is structurally related to a group of [2Fe-2S] ferredoxins involved in iron-sulfur cluster biosynthesis. Comparative genomics suggested that FdVI and orthologs found in alpha-Proteobacteria are involved in this process. Here, the crystal structure of FdVI has been determined for both the oxidized and the reduced protein. The [2Fe-2S] cluster lies 6 A below the protein surface in a hydrophobic pocket without access to the solvent. This particular cluster environment might explain why the FdVI midpoint redox potential (-306 mV at pH 8.0) did not show temperature or ionic strength dependence. Besides the four cysteines that bind the cluster, FdVI features an extra cysteine which is located close to the S1 atom of the cluster and is oriented in a position such that its thiol group points towards the solvent. Upon reduction, the general fold of the polypeptide chain was almost unchanged. The [2Fe-2S] cluster underwent a conformational change from a planar to a distorted lozenge. In the vicinity of the cluster, the side chain of Met24 was rotated by 180 degrees , bringing its S atom within hydrogen-bonding distance of the S2 atom of the cluster. The reduced molecule also featured a higher content of bound water molecules, and more extensive hydrogen-bonding networks compared with the oxidized molecule. The unique conformational changes observed in FdVI upon reduction are discussed in the light of structural studies performed on related ferredoxins.
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(as it appears on PubMed at http://www.pubmed.gov), where 16402206 is the PubMed ID number.
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==About this Structure==
==About this Structure==
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[[Category: Iron-sulfur]]
[[Category: Iron-sulfur]]
[[Category: Metal-binding]]
[[Category: Metal-binding]]
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Revision as of 06:17, 28 July 2008

Image:1uwm.png

Template:STRUCTURE 1uwm

REDUCED FERREDOXIN 6 FROM RHODOBACTER CAPSULATUS

Template:ABSTRACT PUBMED 16402206

About this Structure

1UWM is a Single protein structure of sequence from Rhodobacter capsulatus. Full crystallographic information is available from OCA.

Reference

Structure of a [2Fe-2S] ferredoxin from Rhodobacter capsulatus likely involved in Fe-S cluster biogenesis and conformational changes observed upon reduction., Sainz G, Jakoncic J, Sieker LC, Stojanoff V, Sanishvili N, Asso M, Bertrand P, Armengaud J, Jouanneau Y, J Biol Inorg Chem. 2006 Mar;11(2):235-46. Epub 2006 Jan 10. PMID:16402206

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