1zad

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{{STRUCTURE_1zad| PDB=1zad | SCENE= }}
{{STRUCTURE_1zad| PDB=1zad | SCENE= }}
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'''Structure of cytotoxin I (CTI) from Naja Oxiana in complex with DPC micelle'''
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===Structure of cytotoxin I (CTI) from Naja Oxiana in complex with DPC micelle===
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==Overview==
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The CTs (cytotoxins) I and II are positively charged three-finger folded proteins from venom of Naja oxiana (the Central Asian cobra). They belong to S- and P-type respectively based on Ser-28 and Pro-30 residues within a putative phospholipid bilayer binding site. Previously, we investigated the interaction of CTII with multilamellar liposomes of dipalmitoylphosphatidylglycerol by wide-line (31)P-NMR spectroscopy. To compare interactions of these proteins with phospholipids, we investigated the interaction of CTI with the multilamellar liposomes of dipalmitoylphosphatidylglycerol analogously. The effect of CTI on the chemical shielding anisotropy and deformation of the liposomes in the magnetic field was determined at different temperatures and lipid/protein ratios. It was found that both the proteins do not affect lipid organization in the gel state. In the liquid crystalline state of the bilayer they disturb lipid packing. To get insight into the interactions of the toxins with membranes, Monte Carlo simulations of CTI and CTII in the presence of the bilayer membrane were performed. It was found that both the toxins penetrate into the bilayer with the tips of all the three loops. However, the free-energy gain on membrane insertion of CTI is smaller (by approximately 7 kcal/mol; 1 kcal identical with 4.184 kJ) when compared with CTII, because of the lower hydrophobicity of the membrane-binding site of CTI. These results clearly demonstrate that the P-type cytotoxins interact with membranes stronger than those of the S-type, although the mode of the membrane insertion is similar for both the types.
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(as it appears on PubMed at http://www.pubmed.gov), where 15584897 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15584897}}
==About this Structure==
==About this Structure==
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1ZAD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_oxiana Naja oxiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZAD OCA].
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1ZAD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_oxiana Naja oxiana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZAD OCA].
==Reference==
==Reference==
Interaction of three-finger toxins with phospholipid membranes: comparison of S- and P-type cytotoxins., Dubovskii PV, Lesovoy DM, Dubinnyi MA, Konshina AG, Utkin YN, Efremov RG, Arseniev AS, Biochem J. 2005 May 1;387(Pt 3):807-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15584897 15584897]
Interaction of three-finger toxins with phospholipid membranes: comparison of S- and P-type cytotoxins., Dubovskii PV, Lesovoy DM, Dubinnyi MA, Konshina AG, Utkin YN, Efremov RG, Arseniev AS, Biochem J. 2005 May 1;387(Pt 3):807-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15584897 15584897]
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Comparative study of structure and activity of cytotoxins from venom of the cobras Naja oxiana, Naja kaouthia, and Naja haje., Feofanov AV, Sharonov GV, Dubinnyi MA, Astapova MV, Kudelina IA, Dubovskii PV, Rodionov DI, Utkin YN, Arseniev AS, Biochemistry (Mosc). 2004 Oct;69(10):1148-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15527416 15527416]
[[Category: Naja oxiana]]
[[Category: Naja oxiana]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Beta-turn type i]]
[[Category: Beta-turn type i]]
[[Category: Beta-turn type ii]]
[[Category: Beta-turn type ii]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:17:17 2008''

Revision as of 06:17, 28 July 2008

Image:1zad.png

Template:STRUCTURE 1zad

Structure of cytotoxin I (CTI) from Naja Oxiana in complex with DPC micelle

Template:ABSTRACT PUBMED 15584897

About this Structure

1ZAD is a Single protein structure of sequence from Naja oxiana. Full experimental information is available from OCA.

Reference

Interaction of three-finger toxins with phospholipid membranes: comparison of S- and P-type cytotoxins., Dubovskii PV, Lesovoy DM, Dubinnyi MA, Konshina AG, Utkin YN, Efremov RG, Arseniev AS, Biochem J. 2005 May 1;387(Pt 3):807-15. PMID:15584897

Comparative study of structure and activity of cytotoxins from venom of the cobras Naja oxiana, Naja kaouthia, and Naja haje., Feofanov AV, Sharonov GV, Dubinnyi MA, Astapova MV, Kudelina IA, Dubovskii PV, Rodionov DI, Utkin YN, Arseniev AS, Biochemistry (Mosc). 2004 Oct;69(10):1148-57. PMID:15527416

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