1z8x

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{{STRUCTURE_1z8x| PDB=1z8x | SCENE= }}
{{STRUCTURE_1z8x| PDB=1z8x | SCENE= }}
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'''Structure of Mutant Pyrrolidone Carboxyl Peptidase (E192V) from a Hyperthermophile, Pyrococcus furiosus'''
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===Structure of Mutant Pyrrolidone Carboxyl Peptidase (E192V) from a Hyperthermophile, Pyrococcus furiosus===
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==Overview==
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Pyrrolidone carboxyl peptidases (PCPs) from hyperthermophiles have a structurally conserved and completely buried Glu192 in the hydrophobic core; in contrast, the corresponding residue in the mesophile protein is a hydrophobic residue, Ile. Does the buried ionizable residue contribute to stabilization or destabilization of hyperthermophile PCPs? To elucidate the role of the buried glutamic acid in stabilizing PCP from hyperthermophiles, we constructed five Glu192 mutants of PCP-0SH (C142S/C188S, Cys-free double mutant of PCP) from Pyrococcus furiosus and examined their thermal and pH-induced unfolding and crystal structures and compared them with those of PCP-0SH. The stabilities of apolar (E192A/I/V) and polar (E192D/Q) mutants were less than PCP-0SH at acidic pH values. In the alkaline region, the mutant proteins, except for E192D, were more stable than PCP-0SH. The thermal stability data and theoretical calculations indicated an apparent pKa value &gt; or = 7.3 for Glu192. Present results confirmed that the protonated Glu192 in PCP-0SH forms strong hydrogen bonds with the carbonyl oxygen and peptide nitrogen of Pro168. New intermolecular hydrogen bonds in the E --&gt; A/D mutants were formed by a water molecule introduced into the cavity created around position 192, whereas the hydrogen bonds disappeared in the E --&gt; I/V mutants. Structure-based empirical stability of mutant proteins was in good agreement with the experimental results. The results indicated that (1) completely buried Glu192 contributes to the stabilization of PCP-0SH because of the formation of strong intramolecular hydrogen bonds and (2) the hydrogen bonds by the nonionized and buried Glu can contribute more than the burial of hydrophobic groups to the conformational stability of proteins.
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{{ABSTRACT_PUBMED_16752900}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Completely buried, non-ion-paired glutamic acid contributes favorably to the conformational stability of pyrrolidone carboxyl peptidases from hyperthermophiles., Kaushik JK, Iimura S, Ogasahara K, Yamagata Y, Segawa S, Yutani K, Biochemistry. 2006 Jun 13;45(23):7100-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16752900 16752900]
Completely buried, non-ion-paired glutamic acid contributes favorably to the conformational stability of pyrrolidone carboxyl peptidases from hyperthermophiles., Kaushik JK, Iimura S, Ogasahara K, Yamagata Y, Segawa S, Yutani K, Biochemistry. 2006 Jun 13;45(23):7100-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16752900 16752900]
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X-ray crystalline structures of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus, and its cys-free mutant., Tanaka H, Chinami M, Mizushima T, Ogasahara K, Ota M, Tsukihara T, Yutani K, J Biochem. 2001 Jul;130(1):107-18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11432786 11432786]
[[Category: Pyrococcus furiosus]]
[[Category: Pyrococcus furiosus]]
[[Category: Pyroglutamyl-peptidase I]]
[[Category: Pyroglutamyl-peptidase I]]
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[[Category: Yutani, K.]]
[[Category: Yutani, K.]]
[[Category: Protein stability]]
[[Category: Protein stability]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:19:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:20:18 2008''

Revision as of 06:20, 28 July 2008

Image:1z8x.png

Template:STRUCTURE 1z8x

Structure of Mutant Pyrrolidone Carboxyl Peptidase (E192V) from a Hyperthermophile, Pyrococcus furiosus

Template:ABSTRACT PUBMED 16752900

About this Structure

1Z8X is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

Reference

Completely buried, non-ion-paired glutamic acid contributes favorably to the conformational stability of pyrrolidone carboxyl peptidases from hyperthermophiles., Kaushik JK, Iimura S, Ogasahara K, Yamagata Y, Segawa S, Yutani K, Biochemistry. 2006 Jun 13;45(23):7100-12. PMID:16752900

X-ray crystalline structures of pyrrolidone carboxyl peptidase from a hyperthermophile, Pyrococcus furiosus, and its cys-free mutant., Tanaka H, Chinami M, Mizushima T, Ogasahara K, Ota M, Tsukihara T, Yutani K, J Biochem. 2001 Jul;130(1):107-18. PMID:11432786

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