2otb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2otb.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2otb.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2otb| PDB=2otb | SCENE= }}
{{STRUCTURE_2otb| PDB=2otb | SCENE= }}
-
'''Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state'''
+
===Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state===
-
==Overview==
+
<!--
-
Fluorescent protein (FP) variants that can be reversibly converted between fluorescent and nonfluorescent states have proven to be a catalyst for innovation in the field of fluorescence microscopy. However, the structural basis of the process remains poorly understood. High-resolution structures of a FP derived from Clavularia in both the fluorescent and the light-induced nonfluorescent states reveal that the rapid and complete loss of fluorescence observed upon illumination with 450-nm light results from cis-trans isomerization of the chromophore. The photoinduced change in configuration from the well ordered cis isomer to the highly nonplanar and disordered trans isomer is accompanied by a dramatic rearrangement of internal side chains. Taken together, the structures provide an explanation for the loss of fluorescence upon illumination, the slow light-independent recovery, and the rapid light-induced recovery of fluorescence. The fundamental mechanism appears to be common to all of the photoactivatable and reversibly photoswitchable FPs reported to date.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17420458}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17420458 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17420458}}
==About this Structure==
==About this Structure==
Line 28: Line 32:
[[Category: Beta can]]
[[Category: Beta can]]
[[Category: Fluorescent protein]]
[[Category: Fluorescent protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:36:54 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:33:01 2008''

Revision as of 06:33, 28 July 2008

Image:2otb.png

Template:STRUCTURE 2otb

Crystal structure of a monomeric cyan fluorescent protein in the fluorescent state

Template:ABSTRACT PUBMED 17420458

About this Structure

2OTB is a Single protein structure of sequence from Clavularia sp.. Full crystallographic information is available from OCA.

Reference

Structural basis for reversible photobleaching of a green fluorescent protein homologue., Henderson JN, Ai HW, Campbell RE, Remington SJ, Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6672-7. Epub 2007 Apr 9. PMID:17420458

Page seeded by OCA on Mon Jul 28 09:33:01 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2otb"
Personal tools