This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2hhc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2hhc.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:2hhc.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2hhc| PDB=2hhc | SCENE= }}
{{STRUCTURE_2hhc| PDB=2hhc | SCENE= }}
-
'''Crystal structure of fucosyltransferase NodZ from Bradyrhizobium'''
+
===Crystal structure of fucosyltransferase NodZ from Bradyrhizobium===
-
==Overview==
+
<!--
-
The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17762900}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17762900 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17762900}}
==About this Structure==
==About this Structure==
Line 20: Line 24:
==Reference==
==Reference==
High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17762900 17762900]
High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17762900 17762900]
 +
 +
Cloning, purification, crystallization and preliminary crystallographic studies of Bradyrhizobium fucosyltransferase NodZ., Brzezinski K, Rogozinski B, Stepkowski T, Bujacz G, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):344-6. Epub 2004, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14747720 14747720]
 +
 +
Rhizobium sp. strain NGR234 NodZ protein is a fucosyltransferase., Quesada-Vincens D, Fellay R, Nasim T, Viprey V, Burger U, Prome JC, Broughton WJ, Jabbouri S, J Bacteriol. 1997 Aug;179(16):5087-93. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9260950 9260950]
 +
 +
Bacterial nodulation protein NodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin., Quinto C, Wijfjes AH, Bloemberg GV, Blok-Tip L, Lopez-Lara IM, Lugtenberg BJ, Thomas-Oates JE, Spaink HP, Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4336-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9113990 9113990]
[[Category: Bradyrhizobium sp.]]
[[Category: Bradyrhizobium sp.]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 31: Line 41:
[[Category: Nodulation]]
[[Category: Nodulation]]
[[Category: Nodz]]
[[Category: Nodz]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:17:36 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:33:42 2008''

Revision as of 06:33, 28 July 2008

Image:2hhc.png

Template:STRUCTURE 2hhc

Crystal structure of fucosyltransferase NodZ from Bradyrhizobium

Template:ABSTRACT PUBMED 17762900

About this Structure

2HHC is a Single protein structure of sequence from Bradyrhizobium sp.. Full crystallographic information is available from OCA.

Reference

High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:17762900

Cloning, purification, crystallization and preliminary crystallographic studies of Bradyrhizobium fucosyltransferase NodZ., Brzezinski K, Rogozinski B, Stepkowski T, Bujacz G, Jaskolski M, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):344-6. Epub 2004, Jan 23. PMID:14747720

Rhizobium sp. strain NGR234 NodZ protein is a fucosyltransferase., Quesada-Vincens D, Fellay R, Nasim T, Viprey V, Burger U, Prome JC, Broughton WJ, Jabbouri S, J Bacteriol. 1997 Aug;179(16):5087-93. PMID:9260950

Bacterial nodulation protein NodZ is a chitin oligosaccharide fucosyltransferase which can also recognize related substrates of animal origin., Quinto C, Wijfjes AH, Bloemberg GV, Blok-Tip L, Lopez-Lara IM, Lugtenberg BJ, Thomas-Oates JE, Spaink HP, Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4336-41. PMID:9113990

Page seeded by OCA on Mon Jul 28 09:33:42 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hhc"
Personal tools