2ibn

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[[Image:2ibn.gif|left|200px]]
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{{STRUCTURE_2ibn| PDB=2ibn | SCENE= }}
{{STRUCTURE_2ibn| PDB=2ibn | SCENE= }}
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'''Crystal structure of Human myo-Inositol Oxygenase (MIOX)'''
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===Crystal structure of Human myo-Inositol Oxygenase (MIOX)===
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==Overview==
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Altered inositol metabolism is implicated in a number of diabetic complications. The first committed step in mammalian inositol catabolism is performed by myo-inositol oxygenase (MIOX), which catalyzes a unique four-electron dioxygen-dependent ring cleavage of myo-inositol to d-glucuronate. Here, we present the crystal structure of human MIOX in complex with myo-inosose-1 bound in a terminal mode to the MIOX diiron cluster site. Furthermore, from biochemical and biophysical results from N-terminal deletion mutagenesis we show that the N terminus is important, through coordination of a set of loops covering the active site, in shielding the active site during catalysis. EPR spectroscopy of the unliganded enzyme displays a two-component spectrum that we can relate to an open and a closed active site conformation. Furthermore, based on site-directed mutagenesis in combination with biochemical and biophysical data, we propose a novel role for Lys(127) in governing access to the diiron cluster.
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(as it appears on PubMed at http://www.pubmed.gov), where 18364358 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18364358}}
==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Structural genomics consortium]]
[[Category: Structural genomics consortium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:54:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:39:17 2008''

Revision as of 06:39, 28 July 2008

Image:2ibn.png

Template:STRUCTURE 2ibn

Crystal structure of Human myo-Inositol Oxygenase (MIOX)

Template:ABSTRACT PUBMED 18364358

About this Structure

2IBN is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural and Biophysical Characterization of Human myo-Inositol Oxygenase., Thorsell AG, Persson C, Voevodskaya N, Busam RD, Hammarstrom M, Graslund S, Graslund A, Hallberg BM, J Biol Chem. 2008 May 30;283(22):15209-16. Epub 2008 Mar 24. PMID:18364358

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