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2dwx

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{{STRUCTURE_2dwx| PDB=2dwx | SCENE= }}
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'''Co-crystal Structure Analysis of GGA1-GAE with the WNSF motif'''
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===Co-crystal Structure Analysis of GGA1-GAE with the WNSF motif===
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==Overview==
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Golgi-localizing, gamma-adaptin ear domain homology, ADP ribosylation factor-binding (GGA) proteins and the adaptor protein (AP) complex, AP-1, are involved in membrane traffic between the trans Golgi network and the endosomes. The gamma-adaptin ear (GAE) domain of GGAs and the gamma1 ear domain of AP-1 interact with an acidic phenylalanine motif found in accessory proteins. The GAE domain of GGA1 (GGA1-GAE) interacts with a WNSF-containing peptide derived from its own hinge region, although the peptide sequence deviates from the standard acidic phenylalanine motif. We report here the structure of GGA1-GAE in complex with the GGA1 hinge peptide, which revealed that the two aromatic side chains of the WNSF sequence fit into a hydrophobic groove formed by aliphatic portions of the side chains of conserved arginine and lysine residues of GGA1-GAE, in a similar manner to the interaction between GGA-GAEs and acidic phenylalanine sequences from the accessory proteins. Fluorescence quenching experiments indicate that the GGA1 hinge region binds to GGA1-GAE and competes with accessory proteins for binding. Taken together with the previous observation that gamma1 ear binds to the GGA1 hinge region, the interaction between the hinge region and the GAE domain underlies the autoregulation of GGA function in clathrin-mediated trafficking through competing with the accessory proteins and the AP-1 complex.
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(as it appears on PubMed at http://www.pubmed.gov), where 17506864 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17506864}}
==About this Structure==
==About this Structure==
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[[Category: Adaptin]]
[[Category: Adaptin]]
[[Category: Ig fold]]
[[Category: Ig fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:32:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:47:31 2008''

Revision as of 06:47, 28 July 2008

Image:2dwx.png

Template:STRUCTURE 2dwx

Co-crystal Structure Analysis of GGA1-GAE with the WNSF motif

Template:ABSTRACT PUBMED 17506864

About this Structure

2DWX is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Molecular basis for autoregulatory interaction between GAE domain and hinge region of GGA1., Inoue M, Shiba T, Ihara K, Yamada Y, Hirano S, Kamikubo H, Kataoka M, Kawasaki M, Kato R, Nakayama K, Wakatsuki S, Traffic. 2007 Jul;8(7):904-13. Epub 2007 May 16. PMID:17506864

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