1nej

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{{STRUCTURE_1nej| PDB=1nej | SCENE= }}
{{STRUCTURE_1nej| PDB=1nej | SCENE= }}
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'''Crystalline Human Carbonmonoxy Hemoglobin S (Liganded Sickle Cell Hemoglobin) Exhibits The R2 Quaternary State At Neutral pH In The Presence Of Polyethylene Glycol: The 2.1 Angstrom Resolution Crystal Structure'''
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===Crystalline Human Carbonmonoxy Hemoglobin S (Liganded Sickle Cell Hemoglobin) Exhibits The R2 Quaternary State At Neutral pH In The Presence Of Polyethylene Glycol: The 2.1 Angstrom Resolution Crystal Structure===
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==Overview==
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Human hemoglobin binds oxygen cooperatively and functions as a tetramer composed of two identical alphabeta heterodimers. While human hemoglobin is the best characterized allosteric protein, the quaternary R (oxygenated or liganded) to T (deoxygenated) structural transition remains controversial. The R2 state has been postulated to represent either an intermediate or final quaternary state elicited by ligand binding. However, the biological relevance of the R2 state has been questioned as it has not been observed crystallographically under physiological conditions. The high-resolution R2 quaternary structures of human COHbC (betaE6K) and COHbS (betaE6V) are reported at neutral pH and low ionic strength using PEG 4000 as a precipitant. Crystals of COHbC, COHbS and their mixtures are isomorphous, indicating that they share the same tertiary and quaternary structures. In contrast, oxyHbA or COHbA did not yield crystals at neutral pH under similar conditions. Solubility studies and modeling suggest that at neutral pH and low ionic strength the beta6 mutant hemoglobins crystallize (betaK6 &gt; betaV6) as a result of more favorable lattice contacts.
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(as it appears on PubMed at http://www.pubmed.gov), where 15858266 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15858266}}
==About this Structure==
==About this Structure==
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[[Category: Human hemoglobin]]
[[Category: Human hemoglobin]]
[[Category: R2 quaternary state]]
[[Category: R2 quaternary state]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:26:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:48:46 2008''

Revision as of 06:48, 28 July 2008

Image:1nej.png

Template:STRUCTURE 1nej

Crystalline Human Carbonmonoxy Hemoglobin S (Liganded Sickle Cell Hemoglobin) Exhibits The R2 Quaternary State At Neutral pH In The Presence Of Polyethylene Glycol: The 2.1 Angstrom Resolution Crystal Structure

Template:ABSTRACT PUBMED 15858266

About this Structure

1NEJ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

COHbC and COHbS crystallize in the R2 quaternary state at neutral pH in the presence of PEG 4000., Patskovska LN, Patskovsky YV, Almo SC, Hirsch RE, Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):566-73. Epub 2005, Apr 20. PMID:15858266

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