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2rdq

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[[Image:2rdq.gif|left|200px]]
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{{STRUCTURE_2rdq| PDB=2rdq | SCENE= }}
{{STRUCTURE_2rdq| PDB=2rdq | SCENE= }}
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'''Crystal Structure of PtlH with Fe/alpha ketoglutarate bound'''
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===Crystal Structure of PtlH with Fe/alpha ketoglutarate bound===
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==Overview==
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The non-heme iron dioxygenase PtlH from the soil organism Streptomyces avermitilis is a member of the iron(II)/alpha-ketoglutarate-dependent dioxygenase superfamily and catalyzes an essential reaction in the biosynthesis of the sesquiterpenoid antibiotic pentalenolactone. To investigate the structural basis for substrate recognition and catalysis, we have determined the x-ray crystal structure of PtlH in several complexes with the cofactors iron, alpha-ketoglutarate, and the non-reactive enantiomer of the substrate, ent-1-deoxypentalenic acid, in four different crystal forms to up to 1.31 A resolution. The overall structure of PtlH forms a double-stranded barrel helix fold, and the cofactor-binding site for iron and alpha-ketoglutarate is similar to other double-stranded barrel helix fold enzymes. Additional secondary structure elements that contribute to the substrate-binding site in PtlH are not conserved in other double-stranded barrel helix fold enzymes. Binding of the substrate enantiomer induces a reorganization of the monoclinic crystal lattice leading to a disorder-order transition of a C-terminal alpha-helix. The newly formed helix blocks the major access to the active site and effectively traps the bound substrate. Kinetic analysis of wild type and site-directed mutant proteins confirms a critical function of two arginine residues in substrate binding, while simulated docking of the enzymatic reaction product reveals the likely orientation of bound substrate.
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{{ABSTRACT_PUBMED_17942405}}
==About this Structure==
==About this Structure==
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[[Category: Double stranded barrel helix]]
[[Category: Double stranded barrel helix]]
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 16:42:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:49:43 2008''

Revision as of 06:49, 28 July 2008

Image:2rdq.png

Template:STRUCTURE 2rdq

Crystal Structure of PtlH with Fe/alpha ketoglutarate bound

Template:ABSTRACT PUBMED 17942405

About this Structure

2RDQ is a Single protein structure of sequence from Streptomyces avermitilis. Full crystallographic information is available from OCA.

Reference

Crystal structure of the non-heme iron dioxygenase PtlH in pentalenolactone biosynthesis., You Z, Omura S, Ikeda H, Cane DE, Jogl G, J Biol Chem. 2007 Dec 14;282(50):36552-60. Epub 2007 Oct 16. PMID:17942405

Page seeded by OCA on Mon Jul 28 09:49:43 2008

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