1m9w
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(New page: 200px<br /><applet load="1m9w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m9w" /> '''Study of electrostatic potential surface dis...)
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Revision as of 19:08, 20 November 2007
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Study of electrostatic potential surface distribution using high resolution side-chain conformation determined by NMR
Overview
Plastocyanin is a small (approximately 10 kDa), type I blue copper protein, that works as an electron donor to photosystem I from cytochrome f in both, chloroplast systems and in some strains of cyanobacteria. Comparative, studies of the kinetic mechanisms of plastocyanins in different organisms, show that the electron transfer from photosystem I happens by simple, collision in cyanobacteria but through a intermediate transition complex, in green algae and superior plants. Previous work has proved that this, effect cannot be explained by structural variations across the different, plastocyanins but it can be explained by differences in the electrostatic, potential distribution at the protein surface. In that case, minor, conformational errors at the amino acid side chain level may imply an, important effect in the electrostatic potential distribution calculation., In this work we present a high resolution study of side chain conformation, by homonuclear NMR for the reduced wild-type plastocyanin Synechocystis, using intensity ratios for 2D-NOESY and 2D-H,H-TOCSY cross peaks at, different mixing times. We also present the corresponding comparison with, different plastocyanin structures and the effect in the electrostatic, potential distribution at the protein surface. We discuss the importance, of indirect J-coupling information from TOCSY-type experiments as, complement for intraresidue distances derived from NOESY experiments in, the determination of side chain orientation and stereo-specific, assignments.
About this Structure
1M9W is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.
Reference
Study of electrostatic potential surface distribution of wild-type plastocyanin Synechocystis solution structure determined by homonuclear NMR., Monleon D, Celda B, Biopolymers. 2003 Oct;70(2):212-20. PMID:14517909
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