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2ssp

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{{STRUCTURE_2ssp| PDB=2ssp | SCENE= }}
{{STRUCTURE_2ssp| PDB=2ssp | SCENE= }}
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'''LEUCINE-272-ALANINE URACIL-DNA GLYCOSYLASE BOUND TO ABASIC SITE-CONTAINING DNA'''
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===LEUCINE-272-ALANINE URACIL-DNA GLYCOSYLASE BOUND TO ABASIC SITE-CONTAINING DNA===
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==Overview==
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Three high-resolution crystal structures of DNA complexes with wild-type and mutant human uracil-DNA glycosylase (UDG), coupled kinetic characterizations and comparisons with the refined unbound UDG structure help resolve fundamental issues in the initiation of DNA base excision repair (BER): damage detection, nucleotide flipping versus extrahelical nucleotide capture, avoidance of apurinic/apyrimidinic (AP) site toxicity and coupling of damage-specific and damage-general BER steps. Structural and kinetic results suggest that UDG binds, kinks and compresses the DNA backbone with a 'Ser-Pro pinch' and scans the minor groove for damage. Concerted shifts in UDG simultaneously form the catalytically competent active site and induce further compression and kinking of the double-stranded DNA backbone only at uracil and AP sites, where these nucleotides can flip at the phosphate-sugar junction into a complementary specificity pocket. Unexpectedly, UDG binds to AP sites more tightly and more rapidly than to uracil-containing DNA, and thus may protect cells sterically from AP site toxicity. Furthermore, AP-endonuclease, which catalyzes the first damage-general step of BER, enhances UDG activity, most likely by inducing UDG release via shared minor groove contacts and flipped AP site binding. Thus, AP site binding may couple damage-specific and damage-general steps of BER without requiring direct protein-protein interactions.
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(as it appears on PubMed at http://www.pubmed.gov), where 9724657 is the PubMed ID number.
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==About this Structure==
==About this Structure==
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[[Category: Protein/dna]]
[[Category: Protein/dna]]
[[Category: Uracil]]
[[Category: Uracil]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 17:21:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:06:17 2008''

Revision as of 07:06, 28 July 2008

Image:2ssp.png

Template:STRUCTURE 2ssp

LEUCINE-272-ALANINE URACIL-DNA GLYCOSYLASE BOUND TO ABASIC SITE-CONTAINING DNA

Template:ABSTRACT PUBMED 9724657

About this Structure

2SSP is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Base excision repair initiation revealed by crystal structures and binding kinetics of human uracil-DNA glycosylase with DNA., Parikh SS, Mol CD, Slupphaug G, Bharati S, Krokan HE, Tainer JA, EMBO J. 1998 Sep 1;17(17):5214-26. PMID:9724657

Page seeded by OCA on Mon Jul 28 10:06:17 2008

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