1rpv

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{{STRUCTURE_1rpv| PDB=1rpv | SCENE= }}
{{STRUCTURE_1rpv| PDB=1rpv | SCENE= }}
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'''HIV-1 REV PROTEIN (RESIDUES 34-50)'''
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===HIV-1 REV PROTEIN (RESIDUES 34-50)===
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==Overview==
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NMR spectroscopy has been used to solve the three-dimensional solution structure of a minimal RNA-binding domain of the Rev protein from the human immunodeficiency virus (type 1), an essential regulatory protein for viral replication. The presence of 10 arginine residues in the 17-residue peptide Rev34-50 caused significant problems in assignment of the NMR spectra. To improve spectral resolution, the peptide was synthesized with an alanine replacing a nonessential arginine and with selectively 15N-labeled residues. Contrary to Chou-Fasman modeling predictions an alpha-helix was detected in both water and 20% trifluoroethanol (TFE) and was found to span residues that constitute the RNA-binding and nuclear-localizing domains of Rev. The sequence-specific information provided by the NMR data gives a full description of the solution conformation of Rev34-50 which serves as a template for investigating binding of the peptide to RNA from the Rev response element (RRE). Preliminary modeling suggests that the helix can fit neatly into the expanded major groove of the RRE where interactions between the peptide side chains and the RNA can be identified. These data may aid the construction of a suitable pharmacophore model for the rational design of molecules that block Rev-RNA binding and inhibit HIV replication.
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{{ABSTRACT_PUBMED_7599117}}
==About this Structure==
==About this Structure==
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1RPV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_type_1_(isolate_12) Human immunodeficiency virus type 1 (isolate 12)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RPV OCA].
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1RPV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_type_1_(isolate_12) Human immunodeficiency virus type 1 (isolate 12)]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RPV OCA].
==Reference==
==Reference==
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[[Category: Human immunodeficiency virus-1]]
[[Category: Human immunodeficiency virus-1]]
[[Category: Rev response element]]
[[Category: Rev response element]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:46:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:07:35 2008''

Revision as of 07:07, 28 July 2008

Image:1rpv.png

Template:STRUCTURE 1rpv

HIV-1 REV PROTEIN (RESIDUES 34-50)

Template:ABSTRACT PUBMED 7599117

About this Structure

1RPV is a Single protein structure of sequence from Human immunodeficiency virus type 1 (isolate 12). Full experimental information is available from OCA.

Reference

NMR solution structure of the RNA-binding peptide from human immunodeficiency virus (type 1) Rev., Scanlon MJ, Fairlie DP, Craik DJ, Englebretsen DR, West ML, Biochemistry. 1995 Jul 4;34(26):8242-9. PMID:7599117

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