1xrx

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[[Image:1xrx.gif|left|200px]]
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{{Seed}}
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[[Image:1xrx.png|left|200px]]
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{{STRUCTURE_1xrx| PDB=1xrx | SCENE= }}
{{STRUCTURE_1xrx| PDB=1xrx | SCENE= }}
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'''Crystal structure of a DNA-binding protein'''
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===Crystal structure of a DNA-binding protein===
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==Overview==
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Escherichia coli SeqA binds clusters of transiently hemimethylated GATC sequences and sequesters the origin of replication, oriC, from methylation and premature reinitiation. Besides oriC, SeqA binds and organizes newly synthesized DNA at replication forks. Binding to multiple GATC sites is crucial for the formation of stable SeqA-DNA complexes. Here we report the crystal structure of the oligomerization domain of SeqA (SeqA-N). The structural unit of SeqA-N is a dimer, which oligomerizes to form a filament. Mutations that disrupt filament formation lead to asynchronous DNA replication, but the resulting SeqA dimer can still bind two GATC sites separated from 5 to 34 base pairs. Truncation of the linker between the oligomerization and DNA-binding domains restricts SeqA to bind two GATC sites separated by one or two full turns. We propose a model of a SeqA filament interacting with multiple GATC sites that accounts for both origin sequestration and chromosome organization.
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The line below this paragraph, {{ABSTRACT_PUBMED_15933720}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15933720 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15933720}}
==About this Structure==
==About this Structure==
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[[Category: Left-handed helix]]
[[Category: Left-handed helix]]
[[Category: Protein filament]]
[[Category: Protein filament]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:26:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:10:24 2008''

Revision as of 07:10, 28 July 2008

Image:1xrx.png

Template:STRUCTURE 1xrx

Crystal structure of a DNA-binding protein

Template:ABSTRACT PUBMED 15933720

About this Structure

1XRX is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a SeqA-N filament: implications for DNA replication and chromosome organization., Guarne A, Brendler T, Zhao Q, Ghirlando R, Austin S, Yang W, EMBO J. 2005 Apr 20;24(8):1502-11. Epub 2005 Mar 31. PMID:15933720

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