2bsz

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[[Image:2bsz.gif|left|200px]]
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{{STRUCTURE_2bsz| PDB=2bsz | SCENE= }}
{{STRUCTURE_2bsz| PDB=2bsz | SCENE= }}
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'''STRUCTURE OF MESORHIZOBIUM LOTI ARYLAMINE N-ACETYLTRANSFERASE 1'''
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===STRUCTURE OF MESORHIZOBIUM LOTI ARYLAMINE N-ACETYLTRANSFERASE 1===
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==Overview==
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The arylamine N-acetyltransferase (NAT) enzymes have been found in a broad range of both eukaryotic and prokaryotic organisms. The NAT enzymes catalyse the transfer of an acetyl group from acetyl Co-enzyme A onto the terminal nitrogen of a range of arylamine, hydrazine and arylhydrazine compounds. Recently, several NAT structures have been reported from different prokaryotic sources including Salmonella typhimurium, Mycobacterium smegmatis and Pseudomonas aeruginosa. Bioinformatics analysis of the Mesorhizobium loti genome revealed two NAT paralogues, the first example of multiple NAT isoenzymes in a eubacterial organism. The M. loti NAT 1 enzyme was recombinantly expressed and purified for X-ray crystallographic studies. The purified enzyme was crystallized in 0.5 M Ca(OAc)2, 16% PEG 3350, 0.1 M Tris-HCl pH 8.5 using the sitting-drop vapour-diffusion method. A data set diffracting to 2.0 A was collected from a single crystal at 100 K. The crystal belongs to the orthorhombic spacegroup P2(1)2(1)2(1), with unit-cell parameters a = 53.2, b = 97.3, c = 114.3 A. The structure was refined to a final free-R factor of 24.8%. The structure reveals that despite low sequence homology, M. loti NAT1 shares the common fold as reported in previous NAT structures and exhibits the same catalytic triad of residues (Cys-His-Asp) in the active site.
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{{ABSTRACT_PUBMED_16508079}}
==About this Structure==
==About this Structure==
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[[Category: Complete proteome]]
[[Category: Complete proteome]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:45:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:17:51 2008''

Revision as of 07:17, 28 July 2008

Image:2bsz.png

Template:STRUCTURE 2bsz

STRUCTURE OF MESORHIZOBIUM LOTI ARYLAMINE N-ACETYLTRANSFERASE 1

Template:ABSTRACT PUBMED 16508079

About this Structure

2BSZ is a Single protein structure of sequence from Mesorhizobium loti. Full crystallographic information is available from OCA.

Reference

Structure of Mesorhizobium loti arylamine N-acetyltransferase 1., Holton SJ, Dairou J, Sandy J, Rodrigues-Lima F, Dupret JM, Noble ME, Sim E, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt, 1):14-6. Epub 2004 Dec 24. PMID:16508079

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