1r5x

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{{STRUCTURE_1r5x| PDB=1r5x | SCENE= }}
{{STRUCTURE_1r5x| PDB=1r5x | SCENE= }}
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'''JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome'''
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===JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome===
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==Overview==
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The JAMM (JAB1/MPN/Mov34 metalloenzyme) motif in Rpn11 and Csn5 underlies isopeptidase activities intrinsic to the proteasome and signalosome, respectively. We show here that the archaebacterial protein AfJAMM possesses the key features of a zinc metalloprotease, yet with a distinct fold. The histidine and aspartic acid of the conserved EX(n)HS/THX(7)SXXD motif coordinate a zinc, whereas the glutamic acid hydrogen-bonds an aqua ligand. By analogy to the active site of thermolysin, we predict that the glutamic acid serves as an acid-base catalyst and the second serine stabilizes a tetrahedral intermediate. Mutagenesis of Csn5 confirms these residues are required for Nedd8 isopeptidase activity. The active site-like architecture specified by the JAMM motif motivates structure-based approaches to the study of JAMM domain proteins and the development of therapeutic proteasome and signalosome inhibitors.
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(as it appears on PubMed at http://www.pubmed.gov), where 14737182 is the PubMed ID number.
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{{ABSTRACT_PUBMED_14737182}}
==About this Structure==
==About this Structure==
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[[Category: Signalosome]]
[[Category: Signalosome]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:07:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:19:45 2008''

Revision as of 07:19, 28 July 2008

Image:1r5x.png

Template:STRUCTURE 1r5x

JAMM: A Metalloprotease-like Zinc Site in the Proteasome and Signalosome

Template:ABSTRACT PUBMED 14737182

About this Structure

1R5X is a Single protein structure of sequence from Archaeoglobus fulgidus dsm 4304. Full crystallographic information is available from OCA.

Reference

JAMM: a metalloprotease-like zinc site in the proteasome and signalosome., Ambroggio XI, Rees DC, Deshaies RJ, PLoS Biol. 2004 Jan;2(1):E2. Epub 2003 Nov 24. PMID:14737182

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