1nkf

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[[Image:1nkf.gif|left|200px]]
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[[Image:1nkf.png|left|200px]]
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{{STRUCTURE_1nkf| PDB=1nkf | SCENE= }}
{{STRUCTURE_1nkf| PDB=1nkf | SCENE= }}
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'''CALCIUM-BINDING PEPTIDE, NMR, 30 STRUCTURES'''
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===CALCIUM-BINDING PEPTIDE, NMR, 30 STRUCTURES===
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==Overview==
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A 12-residue peptide AcDKDGDGYISAAENH2 analogous to the third calcium-binding loop of calmodulin strongly coordinates lanthanide ions (K = 10(5) M-1). When metal saturated, the peptide adopts a very rigid structure, the same as in the native protein, with three last residues AAE fixed in the alpha-helical conformation. Therefore, the peptide provides an ideal helix nucleation site for peptide segments attached to its C terminus. NMR and CD investigations of peptide AcDKDGDGYISAAEAAAQNH2 presented in this paper show that residues A13-Q16 form an alpha-helix of very high stability when the La3+ ion is bound to the D1-E12 loop. In fact, the lowest estimates of the helix content in this segment give values of at least 80% at 1 degreesC and 70% at 25 degreesC. This finding is not compatible with existing helix-coil transition theories and helix propagation parameters, s, reported in the literature. We conclude, therefore, that the initial steps of helix propagation are characterized by much larger s values, whereas helix nucleation is even more unfavorable than is believed. In light of our findings, thermodynamics of the nascent alpha-helices is discussed. The problem of CD spectra of very short alpha-helices is also addressed.
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The line below this paragraph, {{ABSTRACT_PUBMED_9927666}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 9927666 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9927666}}
==About this Structure==
==About this Structure==
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1NKF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NKF OCA].
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1NKF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NKF OCA].
==Reference==
==Reference==
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[[Category: Ef hand calcium binding loop]]
[[Category: Ef hand calcium binding loop]]
[[Category: Nmr structure]]
[[Category: Nmr structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:38:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:24:44 2008''

Revision as of 07:24, 28 July 2008

Image:1nkf.png

Template:STRUCTURE 1nkf

CALCIUM-BINDING PEPTIDE, NMR, 30 STRUCTURES

Template:ABSTRACT PUBMED 9927666

About this Structure

1NKF is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Alpha-helix nucleation by a calcium-binding peptide loop., Siedlecka M, Goch G, Ejchart A, Sticht H, Bierzyski A, Proc Natl Acad Sci U S A. 1999 Feb 2;96(3):903-8. PMID:9927666

Page seeded by OCA on Mon Jul 28 10:24:44 2008

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