1s97

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[[Image:1s97.gif|left|200px]]
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{{STRUCTURE_1s97| PDB=1s97 | SCENE= }}
{{STRUCTURE_1s97| PDB=1s97 | SCENE= }}
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'''DPO4 with GT mismatch'''
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===DPO4 with GT mismatch===
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==Overview==
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The ability or inability of a DNA polymerase to extend a mispair directly affects the establishment of genomic mutations. We report here kinetic analyses of the ability of Dpo4, a Y-family polymerase from Sulfolobus solfataricus, to extend from all mispairs opposite a template G or T. Dpo4 is equally inefficient at extending these mispairs, which include, surprisingly, a G.T mispair expected to conform closely to Watson-Crick geometry. To elucidate the basis of this, we solved the structure of Dpo4 bound to G.T-mispaired primer template in the presence of an incoming nucleotide. As a control, we also determined the structure of Dpo4 bound to a matched A-T base pair at the primer terminus. The structures offer a basis for the low efficiency of Dpo4 in extending a G.T mispair: a reverse wobble that deflects the primer 3'-OH away from the incoming nucleotide.
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(as it appears on PubMed at http://www.pubmed.gov), where 15077104 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15077104}}
==About this Structure==
==About this Structure==
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[[Category: G t mismatch]]
[[Category: G t mismatch]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:26:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:27:18 2008''

Revision as of 07:27, 28 July 2008

Image:1s97.png

Template:STRUCTURE 1s97

DPO4 with GT mismatch

Template:ABSTRACT PUBMED 15077104

About this Structure

1S97 is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Dpo4 is hindered in extending a G.T mismatch by a reverse wobble., Trincao J, Johnson RE, Wolfle WT, Escalante CR, Prakash S, Prakash L, Aggarwal AK, Nat Struct Mol Biol. 2004 May;11(5):457-62. Epub 2004 Apr 11. PMID:15077104

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