1mdf
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(New page: 200px<br /><applet load="1mdf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mdf, resolution 2.50Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 19:13, 20 November 2007
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CRYSTAL STRUCTURE OF DhbE IN ABSENCE OF SUBSTRATE
Overview
The synthesis of the catecholic siderophore bacillibactin is accomplished, by the nonribosomal peptide synthetase (NRPS) encoded by the dhb operon., DhbE is responsible for the initial step in bacillibactin synthesis, the, activation of the aryl acid 2,3-dihydroxybenzoate (DHB). The stand-alone, adenylation (A) domain DhbE, the structure of which is presented here, exhibits greatest homology to other NRPS A-domains, acyl-CoA ligases and, luciferases. It's structure is solved in three different states, without, the ligands ATP and DHB (native state), with the product DHB-AMP, (adenylate state) and with the hydrolyzed product AMP and DHB (hydrolyzed, state). The 59.9-kDa protein folds into two domains, with the active site, at the interface between them. In contrast to previous proposals of a, major reorientation of the large and small domains on substrate binding, we observe only local structural rearrangements. The structure of the, phosphate binding loop could be determined, a motif common to many, adenylate-forming enzymes, as well as with bound DHB-adenylate and the, hydrolyzed product DHB*AMP. Based on the structure and amino acid sequence, alignments, an adapted specificity conferring code for aryl acid, activating domains is proposed, allowing assignment of substrate, specificity to gene products of previously unknown function.
About this Structure
1MDF is a Single protein structure of sequence from Bacillus subtilis with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of DhbE, an archetype for aryl acid activating domains of modular nonribosomal peptide synthetases., May JJ, Kessler N, Marahiel MA, Stubbs MT, Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12120-5. Epub 2002 Sep 9. PMID:12221282
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