1x0g

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1x0g.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1x0g.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1x0g| PDB=1x0g | SCENE= }}
{{STRUCTURE_1x0g| PDB=1x0g | SCENE= }}
-
'''Crystal Structure of IscA with the [2Fe-2S] cluster'''
+
===Crystal Structure of IscA with the [2Fe-2S] cluster===
-
==Overview==
+
<!--
-
It has been shown that the so-called scaffold proteins are vital in Fe-S cluster biosynthesis by providing an intermediate site for the assembly of Fe-S clusters. However, since no structural information on such scaffold proteins with bound Fe-S cluster intermediates is available, the structural basis of the core of Fe-S cluster biosynthesis remains poorly understood. Here we report the first Fe-S cluster-bound crystal structure of a scaffold protein, IscA, from Thermosynechococcus elongatus, which carries three strictly conserved cysteine residues. Surprisingly, one partially exposed [2Fe-2S] cluster is coordinated by two conformationally distinct IscA protomers, termed alpha and beta, with asymmetric cysteinyl ligation by Cys37, Cys101, Cys103 from alpha and Cys103 from beta. In the crystal, two alphabeta dimers form an unusual domain-swapped tetramer via central domains of beta protomers. Together with additional biochemical data supporting its physiologically relevant configuration, we propose that the unique asymmetric Fe-S cluster coordination and the resulting distinct conformational stabilities of the two IscA protomers are central to the function of IscA-type Fe-S cluster biosynthetic scaffold.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16730357}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16730357 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16730357}}
==About this Structure==
==About this Structure==
Line 40: Line 44:
[[Category: Tetrameric]]
[[Category: Tetrameric]]
[[Category: Three conserved cy]]
[[Category: Three conserved cy]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:22:31 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:28:26 2008''

Revision as of 07:28, 28 July 2008

Image:1x0g.png

Template:STRUCTURE 1x0g

Crystal Structure of IscA with the [2Fe-2S] cluster

Template:ABSTRACT PUBMED 16730357

About this Structure

1X0G is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

The asymmetric IscA homodimer with an exposed [2Fe-2S] cluster suggests the structural basis of the Fe-S cluster biosynthetic scaffold., Morimoto K, Yamashita E, Kondou Y, Lee SJ, Arisaka F, Tsukihara T, Nakai M, J Mol Biol. 2006 Jun 30;360(1):117-32. Epub 2006 May 15. PMID:16730357

Page seeded by OCA on Mon Jul 28 10:28:26 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1x0g"
Personal tools