2z3h

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{{STRUCTURE_2z3h| PDB=2z3h | SCENE= }}
{{STRUCTURE_2z3h| PDB=2z3h | SCENE= }}
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'''Crystal structure of blasticidin S deaminase (BSD) complexed with deaminohydroxy blasticidin S'''
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===Crystal structure of blasticidin S deaminase (BSD) complexed with deaminohydroxy blasticidin S===
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==Overview==
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The set of blasticidin S (BS) and blasticidin S deaminase (BSD) is a widely used selectable marker for gene transfer experiments. BSD is a member of the cytidine deaminase (CDA) family; it is a zinc-dependent enzyme with three cysteines and one water molecule as zinc ligands. The crystal structures of BSD were determined in six states (i.e. native, substrate-bound, product-bound, cacodylate-bound, substrate-bound E56Q mutant, and R90K mutant). In the structures, the zinc position and coordination structures vary. The substrate-bound structure shows a large positional and geometrical shift of zinc with a double-headed electron density of the substrate that seems to be assigned to the amino and hydroxyl groups of the substrate and product, respectively. In this intermediate-like structure, the steric hindrance of the hydroxyl group pushes the zinc into the triangular plane consisting of three cysteines with a positional shift of approximately 0.6 A, and the fifth ligand water approaches the opposite direction of the substrate with a shift of 0.4 A. Accordingly, the zinc coordination is changed from tetrahedral to trigonal bipyramidal, and its coordination distance is extended between zinc and its intermediate. The shift of zinc and the recruited water is also observed in the structure of the inactivated E56Q mutant. This novel observation is different in two-cysteine cytidine deaminase Escherichia coli CDA and might be essential for the reaction mechanism in BSD, since it is useful for the easy release of the product by charge compensation and for the structural change of the substrate.
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(as it appears on PubMed at http://www.pubmed.gov), where 17959604 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17959604}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structures of blasticidin S deaminase (BSD): implications for dynamic properties of catalytic zinc., Kumasaka T, Yamamoto M, Furuichi M, Nakasako M, Teh AH, Kimura M, Yamaguchi I, Ueki T, J Biol Chem. 2007 Dec 21;282(51):37103-11. Epub 2007 Oct 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17959604 17959604]
Crystal structures of blasticidin S deaminase (BSD): implications for dynamic properties of catalytic zinc., Kumasaka T, Yamamoto M, Furuichi M, Nakasako M, Teh AH, Kimura M, Yamaguchi I, Ueki T, J Biol Chem. 2007 Dec 21;282(51):37103-11. Epub 2007 Oct 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17959604 17959604]
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Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089374 10089374]
[[Category: Aspergillus terreus]]
[[Category: Aspergillus terreus]]
[[Category: Blasticidin-S deaminase]]
[[Category: Blasticidin-S deaminase]]
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[[Category: Tetramer]]
[[Category: Tetramer]]
[[Category: Zinc]]
[[Category: Zinc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:29:55 2008''

Revision as of 07:30, 28 July 2008

Image:2z3h.png

Template:STRUCTURE 2z3h

Crystal structure of blasticidin S deaminase (BSD) complexed with deaminohydroxy blasticidin S

Template:ABSTRACT PUBMED 17959604

About this Structure

2Z3H is a Single protein structure of sequence from Aspergillus terreus. Full crystallographic information is available from OCA.

Reference

Crystal structures of blasticidin S deaminase (BSD): implications for dynamic properties of catalytic zinc., Kumasaka T, Yamamoto M, Furuichi M, Nakasako M, Teh AH, Kimura M, Yamaguchi I, Ueki T, J Biol Chem. 2007 Dec 21;282(51):37103-11. Epub 2007 Oct 23. PMID:17959604

Crystallization and preliminary X-ray diffraction studies of blasticidin S deaminase from Aspergillus terreus., Nakasako M, Kimura M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):547-8. PMID:10089374

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