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1s32

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[[Image:1s32.gif|left|200px]]
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{{STRUCTURE_1s32| PDB=1s32 | SCENE= }}
{{STRUCTURE_1s32| PDB=1s32 | SCENE= }}
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'''Molecular Recognition of the Nucleosomal 'Supergroove''''
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===Molecular Recognition of the Nucleosomal 'Supergroove'===
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==Overview==
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Chromatin is the physiological substrate in all processes involving eukaryotic DNA. By organizing 147 base pairs of DNA into two tight superhelical coils, the nucleosome generates an architecture where DNA regions that are 80 base pairs apart on linear DNA are brought into close proximity, resulting in the formation of DNA "supergrooves." Here, we report the design of a hairpin polyamide dimer that targets one such supergroove. The 2-A crystal structure of the nucleosome-polyamide complex shows that the bivalent "clamp" effectively crosslinks the two gyres of the DNA superhelix, improves positioning of the DNA on the histone octamer, and stabilizes the nucleosome against dissociation. Our findings identify nucleosomal supergrooves as platforms for molecular recognition of condensed eukaryotic DNA. In vivo, supergrooves may foster synergistic protein-protein interactions by bringing two regulatory elements into juxtaposition. Because supergroove formation is independent of the translational position of the DNA on the histone octamer, accurate nucleosome positioning over regulatory elements is not required for supergroove participation in eukaryotic gene regulation.
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The line below this paragraph, {{ABSTRACT_PUBMED_15100411}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15100411 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15100411}}
==About this Structure==
==About this Structure==
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[[Category: Clamp]]
[[Category: Clamp]]
[[Category: Nucleosome dynamic]]
[[Category: Nucleosome dynamic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:14:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:30:15 2008''

Revision as of 07:30, 28 July 2008

Image:1s32.png

Template:STRUCTURE 1s32

Molecular Recognition of the Nucleosomal 'Supergroove'

Template:ABSTRACT PUBMED 15100411

About this Structure

1S32 is a Protein complex structure of sequences from Xenopus laevis. Full crystallographic information is available from OCA.

Reference

Molecular recognition of the nucleosomal "supergroove"., Edayathumangalam RS, Weyermann P, Gottesfeld JM, Dervan PB, Luger K, Proc Natl Acad Sci U S A. 2004 May 4;101(18):6864-9. Epub 2004 Apr 20. PMID:15100411

Page seeded by OCA on Mon Jul 28 10:30:15 2008

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