1mdx

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Revision as of 19:14, 20 November 2007

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spinqualitylabelsall models 1mdx, resolution 1.96Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of ArnB transferase with pyridoxal 5' phosphate

Overview

Lipid A modification with 4-amino-4-deoxy-L-arabinose confers on certain, pathogenic bacteria, such as Salmonella, resistance to cationic, antimicrobial peptides, including those derived from the innate immune, system. ArnB catalysis of amino group transfer from glutamic acid to the, 4"-position of a UDP-linked ketopyranose molecule to form, UDP-4-amino-4-deoxy-L-arabinose represents a key step in the lipid A, modification pathway. Structural and functional studies of the ArnB, aminotransferase were undertaken by combining X-ray crystallography with, biochemical analyses. High-resolution crystal structures were solved for, two native forms and one covalently inhibited form of S. typhimurium ArnB., These structures permitted identification of key residues involved in, substrate binding and catalysis, including a rarely observed nonprolyl cis, peptide bond in the active site.

About this Structure

1MDX is a Single protein structure of sequence from Salmonella typhimurium with PLP, AKG and GOL as ligands. Full crystallographic information is available from OCA.

Reference

Structural studies of Salmonella typhimurium ArnB (PmrH) aminotransferase: a 4-amino-4-deoxy-L-arabinose lipopolysaccharide-modifying enzyme., Noland BW, Newman JM, Hendle J, Badger J, Christopher JA, Tresser J, Buchanan MD, Wright TA, Rutter ME, Sanderson WE, Muller-Dieckmann HJ, Gajiwala KS, Buchanan SG, Structure. 2002 Nov;10(11):1569-80. PMID:12429098

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