1qwn

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[[Image:1qwn.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1qwn| PDB=1qwn | SCENE= }}
{{STRUCTURE_1qwn| PDB=1qwn | SCENE= }}
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'''GOLGI ALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride'''
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===GOLGI ALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride===
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==Overview==
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The family 38 golgi alpha-mannosidase II, thought to cleave mannosidic bonds through a double displacement mechanism involving a reaction intermediate, is a clinically important enzyme involved in glycoprotein processing. The structure of three different covalent glycosyl-enzyme intermediates have been determined to 1.2-A resolution for the Golgi alpha-mannosidase II from Drosophila melanogaster by use of fluorinated sugar analogues, both with the wild-type enzyme and a mutant enzyme in which the acid/base catalyst has been removed. All these structures reveal sugar intermediates bound in a distorted 1S5 skew boat conformation. The similarity of this conformation with that of the substrate in the recently determined structure of the Michaelis complex of a beta-mannanase (Ducros, V. M. A., Zechel, D. L., Murshudov, G. N., Gilbert, H. J., Szabo, L., Stoll, D., Withers, S. G., and Davies, G. J. (2002) Angew. Chem. Int. Ed. Engl. 41, 2824-2827) suggests that these disparate enzymes have recruited common stereoelectronic features in evolving their catalytic mechanisms.
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(as it appears on PubMed at http://www.pubmed.gov), where 12960159 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12960159}}
==About this Structure==
==About this Structure==
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[[Category: N-terminal alpha-beta domain]]
[[Category: N-terminal alpha-beta domain]]
[[Category: Three helix bundle]]
[[Category: Three helix bundle]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:47:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:34:20 2008''

Revision as of 07:34, 28 July 2008

Image:1qwn.png

Template:STRUCTURE 1qwn

GOLGI ALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride

Template:ABSTRACT PUBMED 12960159

About this Structure

1QWN is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates., Numao S, Kuntz DA, Withers SG, Rose DR, J Biol Chem. 2003 Nov 28;278(48):48074-83. Epub 2003 Sep 5. PMID:12960159

Page seeded by OCA on Mon Jul 28 10:34:20 2008

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