1otn

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{{STRUCTURE_1otn| PDB=1otn | SCENE= }}
{{STRUCTURE_1otn| PDB=1otn | SCENE= }}
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'''Calcium-binding mutant of the Internalin B LRR domain'''
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===Calcium-binding mutant of the Internalin B LRR domain===
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==Overview==
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The Listeria monocytogenes protein InlB promotes invasion of mammalian cells through activation of the receptor tyrosine kinase Met. The InlB N-cap, a approximately 40 residue part of the domain that binds Met, was previously observed to bind two calcium ions in a novel and unusually exposed manner. Because subsequent work raised questions about the existence of these calcium-binding sites, we assayed calcium binding in solution to the InlB N-cap. We show that calcium ions are bound with dissociation constants in the low micromolar range at the two identified sites, and that the sites interact with one another. We demonstrate that the calcium ions are not required for structure, and also find that they have no appreciable effect on Met activation or intracellular invasion. Therefore, our results indicate that the sites are fortuitous in InlB, but also suggest that the simple architecture of the sites may be adaptable for protein engineering purposes.
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(as it appears on PubMed at http://www.pubmed.gov), where 15020228 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15020228}}
==About this Structure==
==About this Structure==
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[[Category: Invasion]]
[[Category: Invasion]]
[[Category: Listeria]]
[[Category: Listeria]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:16:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:35:11 2008''

Revision as of 07:35, 28 July 2008

Image:1otn.png

Template:STRUCTURE 1otn

Calcium-binding mutant of the Internalin B LRR domain

Template:ABSTRACT PUBMED 15020228

About this Structure

1OTN is a Single protein structure of sequence from Listeria monocytogenes. Full crystallographic information is available from OCA.

Reference

Characterization of the calcium-binding sites of Listeria monocytogenes InlB., Marino M, Banerjee M, Copp J, Dramsi S, Chapman T, van der Geer P, Cossart P, Ghosh P, Biochem Biophys Res Commun. 2004 Apr 2;316(2):379-86. PMID:15020228

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