2p0j

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{{STRUCTURE_2p0j| PDB=2p0j | SCENE= }}
{{STRUCTURE_2p0j| PDB=2p0j | SCENE= }}
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'''Structure of restriction endonuclease BstYI bound to non-cognate DNA'''
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===Structure of restriction endonuclease BstYI bound to non-cognate DNA===
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==Overview==
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DNA recognition by proteins is essential for specific expression of genes in a living organism. En route to a target DNA site, a protein will often sample noncognate DNA sites through nonspecific protein-DNA interactions, resulting in a variety of conformationally different binding states. We present here the crystal structure of endonuclease BstYI bound to a noncognate DNA. Surprisingly, the structure reveals the enzyme in a "hemispecific" binding state on the pathway between nonspecific and specific recognition. A single base pair change in the DNA abolishes binding of only one monomer, with the second monomer bound specifically. We show that the enzyme binds essentially as a rigid body, and that one end of the DNA is accommodated loosely in the binding cleft while the other end is held tightly. Another intriguing feature of the structure is Ser172, which has a dual role in establishing nonspecific and specific contacts. Taken together, the structure provides a snapshot of an enzyme in a "paused" intermediate state that may be part of a more general mechanism of scanning DNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_17437717}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17437717 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17437717}}
==About this Structure==
==About this Structure==
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[[Category: Restriction endonuclease]]
[[Category: Restriction endonuclease]]
[[Category: Scanning]]
[[Category: Scanning]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:04:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:38:23 2008''

Revision as of 07:38, 28 July 2008

Image:2p0j.png

Template:STRUCTURE 2p0j

Structure of restriction endonuclease BstYI bound to non-cognate DNA

Template:ABSTRACT PUBMED 17437717

About this Structure

2P0J is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

BstYI bound to noncognate DNA reveals a "hemispecific" complex: implications for DNA scanning., Townson SA, Samuelson JC, Bao Y, Xu SY, Aggarwal AK, Structure. 2007 Apr;15(4):449-59. PMID:17437717

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