From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:306d.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:306d.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_306d| PDB=306d | SCENE= }} | | {{STRUCTURE_306d| PDB=306d | SCENE= }} |
| | | | |
| - | '''SIDE-BY-SIDE BINDING OF DISTAMYCIN MOLECULES TO D(ICITACIC)'''
| + | ===SIDE-BY-SIDE BINDING OF DISTAMYCIN MOLECULES TO D(ICITACIC)=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | To understand the recognition interactions between AT-containing alternating DNA and minor groove binding drugs, the crystal structures of the side-by-side binding of two distamycin molecules to the DNA octamers d(ICITACIC)2 and d(ICATATIC)2, referred to here as TA and ATAT, respectively, have been determined at 1.6 A and 2.2 A, respectively. Compared to the previous 2:1 all-IC d(ICICICIC)2-distamycin complex, the substitutions of the I x C base-pairs by the A x T base-pairs enable the interactions of the drug with its natural target to be studied. Both complexes assume side-by-side drug binding, isomorphous to the all IC counterpart in the tetragonal space group P4(1)22 (a = b = 28.03 A, c = 58.04 A and a = b = 27.86 A, c = 58.62 A, respectively). The ATAT complex also crystallized in a new polymorphic monoclinic space group C2 (a = 33.38 A, b = 25.33 A, c = 28.11 A and beta = 120.45 degrees) and was solved at 1.9 A resolution. The structures of the three double drug x DNA complexes are very similar, characterized by systematic hydrogen bonding and van der Waals interactions. Each drug hydrogen bonds with the bases of the proximal DNA strand only and stacks with the sugar moiety, while the side-by-side drugs themselves exhibit pyrrole ring-peptide stacking. The pyrrole-peptide interaction is crucial for the side-by-side binding mode of the distamycin/netropsin family of drugs. The purine-pyrimidine alternation is probably responsible for the striking alternation in the helical and backbone conformations. The structures are conserved between the pure IC complex and the AT substituted complexes but further details of the side-by-side binding to DNA are provided by the 1.6 A resolution structure of TA.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9150404}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9150404 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_9150404}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 27: |
Line 31: |
| | [[Category: Double drug in the minor groove]] | | [[Category: Double drug in the minor groove]] |
| | [[Category: Double helix]] | | [[Category: Double helix]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:14:53 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:39:17 2008'' |
Revision as of 07:39, 28 July 2008
Template:STRUCTURE 306d
SIDE-BY-SIDE BINDING OF DISTAMYCIN MOLECULES TO D(ICITACIC)
Template:ABSTRACT PUBMED 9150404
About this Structure
Full crystallographic information is available from OCA.
Reference
Crystal structures of the side-by-side binding of distamycin to AT-containing DNA octamers d(ICITACIC) and d(ICATATIC)., Chen X, Ramakrishnan B, Sundaralingam M, J Mol Biol. 1997 Apr 18;267(5):1157-70. PMID:9150404
Page seeded by OCA on Mon Jul 28 10:39:17 2008
