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3bb0

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{{STRUCTURE_3bb0| PDB=3bb0 | SCENE= }}
{{STRUCTURE_3bb0| PDB=3bb0 | SCENE= }}
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'''Crystal Structure of a Trapped Phosphate-Intermediate in Vanadium Apochloroperoxidase Catalyzing a Dephosphorylation Reaction'''
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===Crystal Structure of a Trapped Phosphate-Intermediate in Vanadium Apochloroperoxidase Catalyzing a Dephosphorylation Reaction===
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==Overview==
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The crystal structure of the apo form of vanadium chloroperoxidase from Curvularia inaequalis reacted with para-nitrophenylphosphate was determined at a resolution of 1.5 A. The aim of this study was to solve structural details of the dephosphorylation reaction catalyzed by this enzyme. Since the chloroperoxidase is functionally and evolutionary related to several acid phosphatases including human glucose-6-phosphatase and a group of membrane-bound lipid phosphatases, the structure sheds light on the details of the dephosphorylation catalyzed by these enzymes as well. The trapped intermediate found is bound to the active site as a metaphosphate anion PO3-, with its phosphorus atom covalently attached to the Nepsilon2 atom of His496. An apical water molecule is within hydrogen-bonding distance to the phosphorus atom of the metaphosphate, and it is in a perfect position for a nucleophilic attack on the metaphosphate-histidine intermediate to form the inorganic phosphate. This is, to our knowledge, the first structural characterization of a real reaction intermediate of the inorganic phosphate group release in a dephosphorylation reaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 18163651 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18163651}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of a trapped phosphate intermediate in vanadium apochloroperoxidase catalyzing a dephosphorylation reaction., de Macedo-Ribeiro S, Renirie R, Wever R, Messerschmidt A, Biochemistry. 2008 Jan 22;47(3):929-34. Epub 2007 Dec 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18163651 18163651]
Crystal structure of a trapped phosphate intermediate in vanadium apochloroperoxidase catalyzing a dephosphorylation reaction., de Macedo-Ribeiro S, Renirie R, Wever R, Messerschmidt A, Biochemistry. 2008 Jan 22;47(3):929-34. Epub 2007 Dec 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18163651 18163651]
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X-ray structure of a vanadium-containing enzyme: chloroperoxidase from the fungus Curvularia inaequalis., Messerschmidt A, Wever R, Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):392-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8552646 8552646]
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X-ray crystal structures of active site mutants of the vanadium-containing chloroperoxidase from the fungus Curvularia inaequalis., Macedo-Ribeiro S, Hemrika W, Renirie R, Wever R, Messerschmidt A, J Biol Inorg Chem. 1999 Apr;4(2):209-19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10499093 10499093]
[[Category: Chloride peroxidase]]
[[Category: Chloride peroxidase]]
[[Category: Curvularia inaequalis]]
[[Category: Curvularia inaequalis]]
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[[Category: Secreted]]
[[Category: Secreted]]
[[Category: Vanadium]]
[[Category: Vanadium]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:35:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:42:17 2008''

Revision as of 07:42, 28 July 2008

Image:3bb0.png

Template:STRUCTURE 3bb0

Crystal Structure of a Trapped Phosphate-Intermediate in Vanadium Apochloroperoxidase Catalyzing a Dephosphorylation Reaction

Template:ABSTRACT PUBMED 18163651

About this Structure

3BB0 is a Single protein structure of sequence from Curvularia inaequalis. Full crystallographic information is available from OCA.

Reference

Crystal structure of a trapped phosphate intermediate in vanadium apochloroperoxidase catalyzing a dephosphorylation reaction., de Macedo-Ribeiro S, Renirie R, Wever R, Messerschmidt A, Biochemistry. 2008 Jan 22;47(3):929-34. Epub 2007 Dec 29. PMID:18163651

X-ray structure of a vanadium-containing enzyme: chloroperoxidase from the fungus Curvularia inaequalis., Messerschmidt A, Wever R, Proc Natl Acad Sci U S A. 1996 Jan 9;93(1):392-6. PMID:8552646

X-ray crystal structures of active site mutants of the vanadium-containing chloroperoxidase from the fungus Curvularia inaequalis., Macedo-Ribeiro S, Hemrika W, Renirie R, Wever R, Messerschmidt A, J Biol Inorg Chem. 1999 Apr;4(2):209-19. PMID:10499093

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