1mf6
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(New page: 200px<br /><applet load="1mf6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1mf6" /> '''Transducin gamma subunit, C-terminal domain ...)
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Revision as of 19:15, 20 November 2007
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Transducin gamma subunit, C-terminal domain 60-71, rhodopsin-bound state: Ensemble of 15 models determined by TrNOE spectroscopy
Overview
Rhodopsin, a prototypical G protein-coupled receptor, catalyzes the, activation of a heterotrimeric G protein, transducin, to initiate a visual, signaling cascade in photoreceptor cells. The betagamma subunit complex, especially the C-terminal domain of the transducin gamma subunit, Gtgamma(60-71)farnesyl, plays a pivotal role in allosteric regulation of, nucleotide exchange on the transducin alpha subunit by light-activated, rhodopsin. We report that this domain is unstructured in the presence of, an inactive receptor but forms an amphipathic helix upon rhodopsin, activation. A K65E/E66K charge reversal mutant of the gamma subunit has, diminished interactions with the receptor and fails to adopt the helical, conformation. The identification of this conformational switch provides a, mechanism for active GPCR utilization of the betagamma complex in signal, transfer to G proteins.
About this Structure
1MF6 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Rhodopsin controls a conformational switch on the transducin gamma subunit., Kisselev OG, Downs MA, Structure. 2003 Apr;11(4):367-73. PMID:12679015
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