2bap

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{{STRUCTURE_2bap| PDB=2bap | SCENE= }}
{{STRUCTURE_2bap| PDB=2bap | SCENE= }}
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'''Crystal structure of the N-terminal mDia1 Armadillo Repeat Region and Dimerisation Domain in complex with the mDia1 autoregulatory domain (DAD)'''
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===Crystal structure of the N-terminal mDia1 Armadillo Repeat Region and Dimerisation Domain in complex with the mDia1 autoregulatory domain (DAD)===
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==Overview==
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Formins induce the nucleation and polymerisation of unbranched actin filaments via the formin-homology domains 1 and 2. Diaphanous-related formins (Drfs) are regulated by a RhoGTPase-binding domain situated in the amino-terminal (N-terminal) region and a carboxy-terminal Diaphanous-autoregulatory domain (DAD), whose interaction stabilises an autoinhibited inactive conformation. Binding of active Rho releases DAD and activates the catalytic activity of mDia. Here, we report on the interaction of DAD with the regulatory N-terminus of mDia1 (mDia(N)) and its release by Rho*GTP. We have defined the elements required for tight binding and solved the three-dimensional structure of a complex between an mDia(N) construct and DAD by X-ray crystallography. The core DAD region is an alpha-helical peptide, which binds in the most highly conserved region of mDia(N) using mainly hydrophobic interactions. The structure suggests a two-step mechanism for release of autoinhibition whereby Rho*GTP, although having a partially nonoverlapping binding site, displaces DAD by ionic repulsion and steric clashes. We show that Rho*GTP accelerates the dissociation of DAD from the mDia(N)*DAD complex.
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(as it appears on PubMed at http://www.pubmed.gov), where 16292343 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16292343}}
==About this Structure==
==About this Structure==
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[[Category: All helical]]
[[Category: All helical]]
[[Category: Armadillo repeat]]
[[Category: Armadillo repeat]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:03:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:46:17 2008''

Revision as of 07:46, 28 July 2008

Image:2bap.png

Template:STRUCTURE 2bap

Crystal structure of the N-terminal mDia1 Armadillo Repeat Region and Dimerisation Domain in complex with the mDia1 autoregulatory domain (DAD)

Template:ABSTRACT PUBMED 16292343

About this Structure

2BAP is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

The regulation of mDia1 by autoinhibition and its release by Rho*GTP., Lammers M, Rose R, Scrima A, Wittinghofer A, EMBO J. 2005 Dec 7;24(23):4176-87. Epub 2005 Nov 17. PMID:16292343

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