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| | {{STRUCTURE_3bn1| PDB=3bn1 | SCENE= }} | | {{STRUCTURE_3bn1| PDB=3bn1 | SCENE= }} |
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| - | '''Crystal structure of GDP-perosamine synthase'''
| + | ===Crystal structure of GDP-perosamine synthase=== |
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| - | ==Overview==
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| - | Perosamine or 4-amino-4,6-dideoxy- d-mannose is an unusual sugar found in the O-antigens of some Gram-negative bacteria such as Vibrio cholerae O1 (the causative agent of cholera) or Escherichia coli O157:H7 (the leading cause of food-borne illnesses). It and similar deoxysugars are added to the O-antigens of bacteria via the action of glycosyltransferases that employ nucleotide-linked sugars as their substrates. The focus of this report is GDP-perosamine synthase, a PLP-dependent enzyme that catalyzes the last step in the formation of GDP-perosamine, namely, the amination of the sugar C-4'. Here we describe the three-dimensional structure of the enzyme from Caulobacter crescentus determined to a nominal resolution of 1.8 A and refined to an R-factor of 17.9%. The overall fold of the enzyme places it into the well-characterized aspartate aminotransferase superfamily. Each subunit of the dimeric enzyme contains a seven-stranded mixed beta-sheet, a two-stranded antiparallel beta-sheet, and 12 alpha-helices. Amino acid residues from both subunits form the active sites of the GDP-perosamine synthase dimer. Recently, the structure of another PLP-dependent enzyme, GDP-4-keto-6-deoxy- d-mannose-3-dehydratase (or ColD), was determined in our laboratory, and this enzyme employs the same substrate as GDP-perosamine synthase. Unlike GDP-perosamine synthase, however, ColD functions as a dehydratase that removes the sugar C-3' hydroxyl group. By purifying the ColD product and reacting it with purified GDP-perosamine synthase, we have produced a novel GDP-linked sugar, GDP-4-amino-3,4,6-trideoxy- d-mannose. Details describing the X-ray structural investigation of GDP-perosamine synthase and the enzymatic synthesis of GDP-4-amino-3,4,6-trideoxy- d-mannose are presented.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_18247575}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 18247575 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_18247575}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Perosamine]] | | [[Category: Perosamine]] |
| | [[Category: X-ray structure]] | | [[Category: X-ray structure]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:55:21 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:47:47 2008'' |
Revision as of 07:47, 28 July 2008
Template:STRUCTURE 3bn1
Crystal structure of GDP-perosamine synthase
Template:ABSTRACT PUBMED 18247575
About this Structure
3BN1 is a Single protein structure of sequence from Caulobacter vibrioides. Full crystallographic information is available from OCA.
Reference
GDP-Perosamine Synthase: Structural Analysis and Production of a Novel Trideoxysugar(,)., Cook PD, Holden HM, Biochemistry. 2008 Mar 4;47(9):2833-40. Epub 2008 Feb 5. PMID:18247575
Page seeded by OCA on Mon Jul 28 10:47:47 2008
