2hik

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[[Image:2hik.jpg|left|200px]]
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{{Seed}}
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{{STRUCTURE_2hik| PDB=2hik | SCENE= }}
{{STRUCTURE_2hik| PDB=2hik | SCENE= }}
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'''heterotrimeric PCNA sliding clamp'''
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===heterotrimeric PCNA sliding clamp===
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==Overview==
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DNA sliding clamps encircle DNA and provide binding sites for many DNA-processing enzymes. However, it is largely unknown how sliding clamps like proliferating cell nuclear antigen (PCNA) coordinate multistep DNA transactions. We have determined structures of Sulfolobus solfataricus DNA ligase and heterotrimeric PCNA separately by X-ray diffraction and in complex by small-angle X-ray scattering (SAXS). Three distinct PCNA subunits assemble into a protein ring resembling the homotrimeric PCNA of humans but with three unique protein-binding sites. In the absence of nicked DNA, the Sulfolobus solfataricus DNA ligase has an open, extended conformation. When complexed with heterotrimeric PCNA, the DNA ligase binds to the PCNA3 subunit and ligase retains an open, extended conformation. A closed, ring-shaped conformation of ligase catalyzes a DNA end-joining reaction that is strongly stimulated by PCNA. This open-to-closed switch in the conformation of DNA ligase is accommodated by a malleable interface with PCNA that serves as an efficient platform for DNA ligation.
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The line below this paragraph, {{ABSTRACT_PUBMED_17052461}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17052461 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17052461}}
==About this Structure==
==About this Structure==
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[[Category: Pcna sliding clamp]]
[[Category: Pcna sliding clamp]]
[[Category: Processivity factor]]
[[Category: Processivity factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:20:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:53:28 2008''

Revision as of 07:53, 28 July 2008

Image:2hik.png

Template:STRUCTURE 2hik

heterotrimeric PCNA sliding clamp

Template:ABSTRACT PUBMED 17052461

About this Structure

2HIK is a Protein complex structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA., Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T, Mol Cell. 2006 Oct 20;24(2):279-91. PMID:17052461

Page seeded by OCA on Mon Jul 28 10:53:28 2008

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