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1zdh

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[[Image:1zdh.gif|left|200px]]
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{{STRUCTURE_1zdh| PDB=1zdh | SCENE= }}
{{STRUCTURE_1zdh| PDB=1zdh | SCENE= }}
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'''MS2 COAT PROTEIN/RNA COMPLEX'''
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===MS2 COAT PROTEIN/RNA COMPLEX===
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==Overview==
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Crystal structures of two complexes between recombinant MS2 capsids and RNA operator fragments have been determined at 2.7 A resolution. The coat protein of the RNA bacteriophage MS2 is bifunctional; it forms the icosahedral virus shell to protect the viral nucleic acid and it acts as a translational repressor by binding with high specificity to a unique site on the RNA, a single stem-loop structure, containing the initiation codon of the gene for the viral replicase. In order to determine the structure of these protein-RNA complexes, we have used chemically synthesized variants of the stem-loop fragment and soaked them into crystals of recombinant capsids. The RNA stem-loop, as bound to the protein, forms a crescent-like structure and interacts with the surface of the beta-sheet of a coat protein dimer. It makes protein contacts with seven phosphate groups on the 5' side of the stem-loop, with a pyrimidine base at position -5, which stacks onto a tyrosine, and with two exposed adenine bases, one in the loop and one at a bulge in the stem. Replacement of the wild-type uridine with a cytosine at position -5 increases the affinity of the RNA to the dimer significantly. The complex with RNA stem-loop having cytosine at this position differs from that of the wild-type complex mainly by having one extra intramolecular RNA interaction and one extra water-mediated hydrogen bond.
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(as it appears on PubMed at http://www.pubmed.gov), where 9245600 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9245600}}
==About this Structure==
==About this Structure==
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[[Category: Rna-binding]]
[[Category: Rna-binding]]
[[Category: Viral protein capsid]]
[[Category: Viral protein capsid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:29:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 10:57:36 2008''

Revision as of 07:57, 28 July 2008

Image:1zdh.png

Template:STRUCTURE 1zdh

MS2 COAT PROTEIN/RNA COMPLEX

Template:ABSTRACT PUBMED 9245600

About this Structure

1ZDH is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.

Reference

The three-dimensional structures of two complexes between recombinant MS2 capsids and RNA operator fragments reveal sequence-specific protein-RNA interactions., Valegard K, Murray JB, Stonehouse NJ, van den Worm S, Stockley PG, Liljas L, J Mol Biol. 1997 Aug 1;270(5):724-38. PMID:9245600

Page seeded by OCA on Mon Jul 28 10:57:36 2008

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