2g6e

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{{STRUCTURE_2g6e| PDB=2g6e | SCENE= }}
{{STRUCTURE_2g6e| PDB=2g6e | SCENE= }}
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'''Structure of cyclized F64L S65A Y66S GFP variant'''
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===Structure of cyclized F64L S65A Y66S GFP variant===
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==Overview==
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The green fluorescent protein (GFP) creates a fluorophore out of three sequential amino acids by promoting spontaneous posttranslational modifications. Here, we use high-resolution crystallography to characterize GFP variants that not only undergo peptide backbone cyclization but additional denaturation-induced peptide backbone fragmentation, native peptide hydrolysis, and decarboxylation reactions. Our analyses indicate that architectural features that favor GFP peptide cyclization also drive peptide hydrolysis. These results are relevant for the maturation pathways of GFP homologues, such as the kindling fluorescent protein and the Kaede protein, which use backbone cleavage to red-shift the spectral properties of their chromophores. We further propose a photochemical mechanism for the decarboxylation reaction, supporting a role for the GFP protein environment in facilitating radical formation and one-electron chemistry, which may be important in activating oxygen for the oxidation step of chromophore biosynthesis. Together, our results characterize GFP posttranslational modification chemistry with implications for the energetic landscape of backbone cyclization and subsequent reactions, and for the rational design of predetermined spontaneous backbone cyclization and cleavage reactions.
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{{ABSTRACT_PUBMED_16594705}}
==About this Structure==
==About this Structure==
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[[Category: Mio]]
[[Category: Mio]]
[[Category: Post-translational modification]]
[[Category: Post-translational modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:44:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:06:15 2008''

Revision as of 08:06, 28 July 2008

Image:2g6e.png

Template:STRUCTURE 2g6e

Structure of cyclized F64L S65A Y66S GFP variant

Template:ABSTRACT PUBMED 16594705

About this Structure

2G6E is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Understanding GFP posttranslational chemistry: structures of designed variants that achieve backbone fragmentation, hydrolysis, and decarboxylation., Barondeau DP, Kassmann CJ, Tainer JA, Getzoff ED, J Am Chem Soc. 2006 Apr 12;128(14):4685-93. PMID:16594705

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