1x3u

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[[Image:1x3u.gif|left|200px]]
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{{Seed}}
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[[Image:1x3u.png|left|200px]]
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{{STRUCTURE_1x3u| PDB=1x3u | SCENE= }}
{{STRUCTURE_1x3u| PDB=1x3u | SCENE= }}
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'''Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium melilot'''
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===Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium melilot===
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==Overview==
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FixJ is a response regulator of the two-component signal transduction pathway involved in the transcriptional activation of nitrogen fixation genes of Sinorhizobium meliloti. Upon phosphorylation, FixJ transcriptionally activates the fixK and nifA promoters. We identified a FixJ recognition sequence of 16 bp in the high affinity binding site of the fixK promoter by means of a gel shift assay. In addition, the solution structure of the truncated C-terminal DNA binding domain of FixJ (FixJC) was solved by NMR spectroscopy. FixJC contains five alpha-helices that encode a typical helix-turn-helix motif as a potential DNA binding core with the highest structural similarity toward the C-terminal DNA binding domain of NarL. The addition of the DNA fragment containing the recognition sequence of the high affinity FixJ binding site resulted in intermediate to slow exchange interactions on the NMR time scale in the spectrum of FixJC, while the exchange was rapid in the case of control DNA. These spectral data suggest that more than one molecule of FixJC binds to the recognition sequence, although FixJC alone is present in monomeric form in solution. This result is consistent with a scenario in which a transcriptionally active species of FixJ is a homodimer of the phosphorylated form.
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{{ABSTRACT_PUBMED_16274231}}
==About this Structure==
==About this Structure==
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1X3U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X3U OCA].
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1X3U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sinorhizobium_meliloti Sinorhizobium meliloti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X3U OCA].
==Reference==
==Reference==
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[[Category: Tamura, K.]]
[[Category: Tamura, K.]]
[[Category: Helix-turn-helix]]
[[Category: Helix-turn-helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:29:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:08:10 2008''

Revision as of 08:08, 28 July 2008

Image:1x3u.png

Template:STRUCTURE 1x3u

Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium melilot

Template:ABSTRACT PUBMED 16274231

About this Structure

1X3U is a Single protein structure of sequence from Sinorhizobium meliloti. Full experimental information is available from OCA.

Reference

Solution structure of the C-terminal transcriptional activator domain of FixJ from Sinorhizobium meliloti and its recognition of the fixK promoter., Kurashima-Ito K, Kasai Y, Hosono K, Tamura K, Oue S, Isogai M, Ito Y, Nakamura H, Shiro Y, Biochemistry. 2005 Nov 15;44(45):14835-44. PMID:16274231

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