2axc

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{{STRUCTURE_2axc| PDB=2axc | SCENE= }}
{{STRUCTURE_2axc| PDB=2axc | SCENE= }}
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'''Crystal structure of ColE7 translocation domain'''
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===Crystal structure of ColE7 translocation domain===
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==Overview==
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ColE7 is a nuclease-type colicin released from Escherichia coli to kill sensitive bacterial cells by degrading the nucleic acid molecules in their cytoplasm. ColE7 is classified as one of the group A colicins, since the N-terminal translocation domain (T-domain) of the nuclease-type colicins interact with specific membrane-bound or periplasmic Tol proteins during protein import. Here, we show that if the N-terminal tail of ColE7 is deleted, ColE7 (residues 63-576) loses its bactericidal activity against E.coli. Moreover, TolB protein interacts directly with the T-domain of ColE7 (residues 1-316), but not with the N-terminal deleted T-domain (residues 60-316), as detected by co-immunoprecipitation experiments, confirming that the N-terminal tail is required for ColE7 interactions with TolB. The crystal structure of the N-terminal tail deleted ColE7 T-domain was determined by the multi-wavelength anomalous dispersion method at a resolution of 1.7 angstroms. The structure of the ColE7 T-domain superimposes well with the T-domain of ColE3 and TR-domain of ColB, a group A Tol-dependent colicin and a group B TonB-dependent colicin, respectively. The structural resemblance of group A and B colicins implies that the two groups of colicins may share a mechanistic connection during cellular import.
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(as it appears on PubMed at http://www.pubmed.gov), where 16360169 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16360169}}
==About this Structure==
==About this Structure==
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[[Category: Colicin]]
[[Category: Colicin]]
[[Category: Translocation domain]]
[[Category: Translocation domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:35:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:11:58 2008''

Revision as of 08:12, 28 July 2008

Image:2axc.png

Template:STRUCTURE 2axc

Crystal structure of ColE7 translocation domain

Template:ABSTRACT PUBMED 16360169

About this Structure

2AXC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranes., Cheng YS, Shi Z, Doudeva LG, Yang WZ, Chak KF, Yuan HS, J Mol Biol. 2006 Feb 10;356(1):22-31. Epub 2005 Dec 5. PMID:16360169

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