1rzr

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1rzr.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1rzr.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1rzr| PDB=1rzr | SCENE= }}
{{STRUCTURE_1rzr| PDB=1rzr | SCENE= }}
-
'''crystal structure of transcriptional regulator-phosphoprotein-DNA complex'''
+
===crystal structure of transcriptional regulator-phosphoprotein-DNA complex===
-
==Overview==
+
<!--
-
Carbon catabolite repression (CCR) is one of the most fundamental environmental-sensing mechanisms in bacteria and imparts competitive advantage by establishing priorities in carbon metabolism. In gram-positive bacteria, the master transcription regulator of CCR is CcpA. CcpA is a LacI-GalR family member that employs, as an allosteric corepressor, the phosphoprotein HPr-Ser46-P, which is formed in glucose-replete conditions. Here we report structures of the Bacillus megaterium apoCcpA and a CcpA-(HPr-Ser46-P)-DNA complex. These structures reveal that HPr-Ser46-P mediates a novel two-component allosteric DNA binding activation mechanism that involves both rotation of the CcpA subdomains and relocation of pivot-point residue Thr61, which leads to juxtaposition of the DNA binding regions permitting "hinge" helix formation in the presence of cognate DNA. The structure of the CcpA-(HPr-Ser46-P)-cre complex also reveals the elegant mechanism by which CcpA family-specific interactions with HPr-Ser46-P residues Ser46-P and His15 partition the high-energy CCR and low-energy PTS pathways, the latter requiring HPr-His15-P.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15369672}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15369672 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15369672}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Phospho-protein]]
[[Category: Phospho-protein]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:06:53 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:12:10 2008''

Revision as of 08:12, 28 July 2008

Image:1rzr.png

Template:STRUCTURE 1rzr

crystal structure of transcriptional regulator-phosphoprotein-DNA complex

Template:ABSTRACT PUBMED 15369672

About this Structure

1RZR is a Protein complex structure of sequences from Bacillus megaterium. Full crystallographic information is available from OCA.

Reference

Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P., Schumacher MA, Allen GS, Diel M, Seidel G, Hillen W, Brennan RG, Cell. 2004 Sep 17;118(6):731-41. PMID:15369672

Page seeded by OCA on Mon Jul 28 11:12:10 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rzr"
Personal tools