This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1y4c

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1y4c.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1y4c.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1y4c| PDB=1y4c | SCENE= }}
{{STRUCTURE_1y4c| PDB=1y4c | SCENE= }}
-
'''Designed Helical Protein fusion MBP'''
+
===Designed Helical Protein fusion MBP===
-
==Overview==
+
<!--
-
An IL-4 antagonist was designed based on structural and biochemical analysis of unbound IL-4 and IL-4 in complex with its high-affinity receptor (IL-4Ralpha). Our design strategy sought to capture a protein-protein interaction targeting the high affinity that IL-4 has for IL-4Ralpha. This strategy has impact due to the potential relevance of IL-4Ralpha as a drug target in the treatment of asthma. To mimic the IL-4 binding surface, critical side chains for receptor binding were identified, and these side chains were transplanted onto a previously characterized, de novo-designed four-helix protein called designed helical protein 1 (DHP-1). This first-generation design resolved the ambiguity previously described for the connectivity between helices in DHP-1 and resulted in a protein capable of binding to IL-4Ralpha. The second-generation antagonist was based upon further molecular modeling, and it succeeded in binding IL-4Ralpha better than the first-generation. This protein, termed DHP-14-AB, yielded a protein with a cooperative unfolding transition (DeltaGu0=8.1 kcal/mol) and an IC50 of 27 microM when in competition with IL-4 whereas DHP-1 had no affinity for IL-4Ralpha. The crystal structure of DHP-14-AB was determined to 1.9-A resolution and was compared with IL-4. This comparison revealed how design strategies targeting protein-protein interactions require high-resolution 3D data and the incorporation of orientation-specific information at the level of side-chains and secondary structure element interactions.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15684085}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15684085 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15684085}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: De novo designed helical protein]]
[[Category: De novo designed helical protein]]
[[Category: Maltose binding protein fusion]]
[[Category: Maltose binding protein fusion]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:51:51 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:24:03 2008''

Revision as of 08:24, 28 July 2008

Image:1y4c.png

Template:STRUCTURE 1y4c

Designed Helical Protein fusion MBP

Template:ABSTRACT PUBMED 15684085

About this Structure

1Y4C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

De novo design of an IL-4 antagonist and its structure at 1.9 A., Laporte SL, Forsyth CM, Cunningham BC, Miercke LJ, Akhavan D, Stroud RM, Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):1889-94. Epub 2005 Jan 31. PMID:15684085

Page seeded by OCA on Mon Jul 28 11:24:03 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1y4c"
Personal tools