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1ye6

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{{STRUCTURE_1ye6| PDB=1ye6 | SCENE= }}
{{STRUCTURE_1ye6| PDB=1ye6 | SCENE= }}
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'''Crystal structure of the Lys-274 to Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NADP+'''
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===Crystal structure of the Lys-274 to Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NADP+===
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==Overview==
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Aldo-keto reductases of family 2 employ single site replacement Lys--&gt;Arg to switch their cosubstrate preference from NADPH to NADH. X-ray crystal structures of Lys-274--&gt;Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+ and NADP+ were determined at a resolution of 2.4 and 2.3A, respectively. Due to steric conflicts in the NADP+-bound form, the arginine side chain must rotate away from the position of the original lysine side chain, thereby disrupting a network of direct and water-mediated interactions between Glu-227, Lys-274 and the cofactor 2'-phosphate and 3'-hydroxy groups. Because anchoring contacts of its Glu-227 are lost, the coenzyme-enfolding loop that becomes ordered upon binding of NAD(P)+ in the wild-type remains partly disordered in the NADP+-bound mutant. The results delineate a catalytic reaction profile for the mutant in comparison to wild-type.
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{{ABSTRACT_PUBMED_15670843}}
==About this Structure==
==About this Structure==
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[[Category: Wilson, D K.]]
[[Category: Wilson, D K.]]
[[Category: Beta-alpha-barrel akr aldo-keto reductase coenzyme specificity nadp]]
[[Category: Beta-alpha-barrel akr aldo-keto reductase coenzyme specificity nadp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:12:53 2008''
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Revision as of 08:25, 28 July 2008

Image:1ye6.png

Template:STRUCTURE 1ye6

Crystal structure of the Lys-274 to Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NADP+

Template:ABSTRACT PUBMED 15670843

About this Structure

1YE6 is a Single protein structure of sequence from Candida tenuis. Full crystallographic information is available from OCA.

Reference

Fine tuning of coenzyme specificity in family 2 aldo-keto reductases revealed by crystal structures of the Lys-274-->Arg mutant of Candida tenuis xylose reductase (AKR2B5) bound to NAD+ and NADP+., Leitgeb S, Petschacher B, Wilson DK, Nidetzky B, FEBS Lett. 2005 Jan 31;579(3):763-7. PMID:15670843

Page seeded by OCA on Mon Jul 28 11:25:04 2008

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