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2i74

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{{STRUCTURE_2i74| PDB=2i74 | SCENE= }}
{{STRUCTURE_2i74| PDB=2i74 | SCENE= }}
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'''Crystal structure of mouse Peptide N-Glycanase C-terminal domain in complex with mannopentaose'''
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===Crystal structure of mouse Peptide N-Glycanase C-terminal domain in complex with mannopentaose===
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==Overview==
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The inability of certain N-linked glycoproteins to adopt their native conformation in the endoplasmic reticulum (ER) leads to their retrotranslocation into the cytosol and subsequent degradation by the proteasome. In this pathway the cytosolic peptide-N-glycanase (PNGase) cleaves the N-linked glycan chains off denatured glycoproteins. PNGase is highly conserved in eukaryotes and plays an important role in ER-associated protein degradation. In higher eukaryotes, PNGase has an N-terminal and a C-terminal extension in addition to its central catalytic domain, which is structurally and functionally related to transglutaminases. Although the N-terminal domain of PNGase is involved in protein-protein interactions, the function of the C-terminal domain has not previously been characterized. Here, we describe biophysical, biochemical, and crystallographic studies of the mouse PNGase C-terminal domain, including visualization of a complex between this domain and mannopentaose. These studies demonstrate that the C-terminal domain binds to the mannose moieties of N-linked oligosaccharide chains, and we further show that it enhances the activity of the mouse PNGase core domain, presumably by increasing the affinity of mouse PNGase for the glycan chains of misfolded glycoproteins.
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==About this Structure==
==About this Structure==
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[[Category: Zhou, X.]]
[[Category: Zhou, X.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:09:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:25:11 2008''

Revision as of 08:25, 28 July 2008

Image:2i74.png

Template:STRUCTURE 2i74

Crystal structure of mouse Peptide N-Glycanase C-terminal domain in complex with mannopentaose

Template:ABSTRACT PUBMED 17088551

About this Structure

2I74 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural and biochemical studies of the C-terminal domain of mouse peptide-N-glycanase identify it as a mannose-binding module., Zhou X, Zhao G, Truglio JJ, Wang L, Li G, Lennarz WJ, Schindelin H, Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17214-9. Epub 2006 Nov 6. PMID:17088551

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