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2dbt

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{{STRUCTURE_2dbt| PDB=2dbt | SCENE= }}
{{STRUCTURE_2dbt| PDB=2dbt | SCENE= }}
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'''Crystal structure of chitinase C from Streptomyces griseus HUT6037'''
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===Crystal structure of chitinase C from Streptomyces griseus HUT6037===
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==Overview==
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Chitinase C (ChiC) from Streptomyces griseus HUT6037 was the first glycoside hydrolase family 19 chitinase that was found in an organism other than higher plants. An N-terminal chitin-binding domain and a C-terminal catalytic domain connected by a linker peptide constitute ChiC. We determined the crystal structure of full-length ChiC, which is the only representative of the two-domain chitinases in the family. The catalytic domain has an alpha-helix-rich fold with a deep cleft containing a catalytic site, and lacks three loops on the domain surface compared with the catalytic domain of plant chitinases. The chitin-binding domain is an all-beta protein with two tryptophan residues (Trp59 and Trp60) aligned on the surface. We suggest the binding mechanism of tri-N-acetylchitotriose onto the chitin-binding domain on the basis of molecular dynamics (MD) simulations. In this mechanism, the ligand molecule binds well on the surface-exposed binding site through two stacking interactions and two hydrogen bonds and only Trp59 and Trp60 are involved in the binding. Furthermore, the flexibility of the Trp60 side-chain, which may be involved in adjusting the binding surface to fit the surface of crystalline chitin by the rotation of chi2 angle, is shown.
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(as it appears on PubMed at http://www.pubmed.gov), where 16516924 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16516924}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16516924 16516924]
Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16516924 16516924]
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Functional analysis of the chitin-binding domain of a family 19 chitinase from Streptomyces griseus HUT6037: substrate-binding affinity and cis-dominant increase of antifungal function., Itoh Y, Kawase T, Nikaidou N, Fukada H, Mitsutomi M, Watanabe T, Itoh Y, Biosci Biotechnol Biochem. 2002 May;66(5):1084-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12092819 12092819]
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Family 19 chitinases of Streptomyces species: characterization and distribution., Watanabe T, Kanai R, Kawase T, Tanabe T, Mitsutomi M, Sakuda S, Miyashita K, Microbiology. 1999 Dec;145 ( Pt 12):3353-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10627034 10627034]
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A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037., Ohno T, Armand S, Hata T, Nikaidou N, Henrissat B, Mitsutomi M, Watanabe T, J Bacteriol. 1996 Sep;178(17):5065-70. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8752320 8752320]
[[Category: Chitinase]]
[[Category: Chitinase]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Watanabe, T.]]
[[Category: Watanabe, T.]]
[[Category: Family 19 chitinase]]
[[Category: Family 19 chitinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:26:01 2008''

Revision as of 08:26, 28 July 2008

Image:2dbt.png

Template:STRUCTURE 2dbt

Crystal structure of chitinase C from Streptomyces griseus HUT6037

Template:ABSTRACT PUBMED 16516924

About this Structure

2DBT is a Single protein structure of sequence from Streptomyces griseus. Full crystallographic information is available from OCA.

Reference

Structural studies of a two-domain chitinase from Streptomyces griseus HUT6037., Kezuka Y, Ohishi M, Itoh Y, Watanabe J, Mitsutomi M, Watanabe T, Nonaka T, J Mol Biol. 2006 Apr 28;358(2):472-84. Epub 2006 Feb 21. PMID:16516924

Functional analysis of the chitin-binding domain of a family 19 chitinase from Streptomyces griseus HUT6037: substrate-binding affinity and cis-dominant increase of antifungal function., Itoh Y, Kawase T, Nikaidou N, Fukada H, Mitsutomi M, Watanabe T, Itoh Y, Biosci Biotechnol Biochem. 2002 May;66(5):1084-92. PMID:12092819

Family 19 chitinases of Streptomyces species: characterization and distribution., Watanabe T, Kanai R, Kawase T, Tanabe T, Mitsutomi M, Sakuda S, Miyashita K, Microbiology. 1999 Dec;145 ( Pt 12):3353-63. PMID:10627034

A modular family 19 chitinase found in the prokaryotic organism Streptomyces griseus HUT 6037., Ohno T, Armand S, Hata T, Nikaidou N, Henrissat B, Mitsutomi M, Watanabe T, J Bacteriol. 1996 Sep;178(17):5065-70. PMID:8752320

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