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2f3o

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{{STRUCTURE_2f3o| PDB=2f3o | SCENE= }}
{{STRUCTURE_2f3o| PDB=2f3o | SCENE= }}
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'''Crystal Structure of a glycyl radical enzyme from Archaeoglobus fulgidus'''
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===Crystal Structure of a glycyl radical enzyme from Archaeoglobus fulgidus===
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==Overview==
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We have solved the crystal structure of a PFL2 from Archaeglobus fulgidus at 2.9 A resolution. Of the three previously solved enzyme structures of glycyl radical enzymes, pyruvate formate lyase (PFL), anaerobic ribonucleotide reductase and glycerol dehydratase (GD), the last one is clearly most similar to PFL2. We observed electron density in the active site of PFL2, which we modelled as glycerol. The orientation of the glycerol is different from that in GD, and changes in the active site indicate that the actual substrate of PFL2 is bigger than a glycerol molecule, but sequence and structural homology suggest that PFL2 may be a dehydratase. Crystal packing, solution X-ray scattering and ultracentrifugation experiments show that PFL2 is tetrameric, unlike other glycyl radical enzymes. A.fulgidus is a hyperthermophile and PFL2 appears to be stabilized by several factors including an increased number of ion pairs, differences in buried charges, a truncated N terminus, anchoring of loops and N terminus via salt-bridges, changes in the oligomeric interface and perhaps also the higher oligomerization state of the protein.
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(as it appears on PubMed at http://www.pubmed.gov), where 16414072 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16414072}}
==About this Structure==
==About this Structure==
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[[Category: Pfl2]]
[[Category: Pfl2]]
[[Category: Pyruvate formate lyase]]
[[Category: Pyruvate formate lyase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:25:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:31:12 2008''

Revision as of 08:31, 28 July 2008

Image:2f3o.png

Template:STRUCTURE 2f3o

Crystal Structure of a glycyl radical enzyme from Archaeoglobus fulgidus

Template:ABSTRACT PUBMED 16414072

About this Structure

2F3O is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.

Reference

Crystal structure of a glycyl radical enzyme from Archaeoglobus fulgidus., Lehtio L, Grossmann JG, Kokona B, Fairman R, Goldman A, J Mol Biol. 2006 Mar 17;357(1):221-35. Epub 2006 Jan 3. PMID:16414072

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