2crx

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{{STRUCTURE_2crx| PDB=2crx | SCENE= }}
{{STRUCTURE_2crx| PDB=2crx | SCENE= }}
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'''STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION'''
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===STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION===
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==Overview==
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We have determined the X-ray crystal structures of two DNA Holliday junctions (HJs) bound by Cre recombinase. The HJ is a four-way branched structure that occurs as an intermediate in genetic recombination pathways, including site-specific recombination by the lambda-integrase family. Cre recombinase is an integrase family member that recombines 34 bp loxP sites in the absence of accessory proteins or auxiliary DNA sequences. The 2.7 A structure of Cre recombinase bound to an immobile HJ and the 2.5 A structure of Cre recombinase bound to a symmetric, nicked HJ reveal a nearly planar, twofold-symmetric DNA intermediate that shares features with both the stacked-X and the square conformations of the HJ that exist in the unbound state. The structures support a protein-mediated crossover isomerization of the junction that acts as the switch responsible for activation and deactivation of recombinase active sites. In this model, a subtle isomerization of the Cre recombinase-HJ quaternary structure dictates which strands are cleaved during resolution of the junction via a mechanism that involves neither branch migration nor helical restacking.
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{{ABSTRACT_PUBMED_9670032}}
==About this Structure==
==About this Structure==
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[[Category: Recombinase/dna complex]]
[[Category: Recombinase/dna complex]]
[[Category: Recombination]]
[[Category: Recombination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:54:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:38:32 2008''

Revision as of 08:38, 28 July 2008

Image:2crx.png

Template:STRUCTURE 2crx

STRUCTURE OF THE HOLLIDAY JUNCTION INTERMEDIATE IN CRE-LOXP SITE-SPECIFIC RECOMBINATION

Template:ABSTRACT PUBMED 9670032

About this Structure

2CRX is a Single protein structure of sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.

Reference

Structure of the Holliday junction intermediate in Cre-loxP site-specific recombination., Gopaul DN, Guo F, Van Duyne GD, EMBO J. 1998 Jul 15;17(14):4175-87. PMID:9670032

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