2b3q

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[[Image:2b3q.gif|left|200px]]
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{{STRUCTURE_2b3q| PDB=2b3q | SCENE= }}
{{STRUCTURE_2b3q| PDB=2b3q | SCENE= }}
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'''Crystal structure of a well-folded variant of green fluorescent protein'''
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===Crystal structure of a well-folded variant of green fluorescent protein===
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==Overview==
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Existing variants of green fluorescent protein (GFP) often misfold when expressed as fusions with other proteins. We have generated a robustly folded version of GFP, called 'superfolder' GFP, that folds well even when fused to poorly folded polypeptides. Compared to 'folding reporter' GFP, a folding-enhanced GFP containing the 'cycle-3' mutations and the 'enhanced GFP' mutations F64L and S65T, superfolder GFP shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics. The fluorescence of Escherichia coli cells expressing each of eighteen proteins from Pyrobaculum aerophilum as fusions with superfolder GFP was proportional to total protein expression. In contrast, fluorescence of folding reporter GFP fusion proteins was strongly correlated with the productive folding yield of the passenger protein. X-ray crystallographic structural analyses helped explain the enhanced folding of superfolder GFP relative to folding reporter GFP.
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(as it appears on PubMed at http://www.pubmed.gov), where 16369541 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16369541}}
==About this Structure==
==About this Structure==
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[[Category: Waldo, G S.]]
[[Category: Waldo, G S.]]
[[Category: 11-stranded beta-barrel]]
[[Category: 11-stranded beta-barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:48:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 12:01:38 2008''

Revision as of 09:01, 28 July 2008

Image:2b3q.png

Template:STRUCTURE 2b3q

Crystal structure of a well-folded variant of green fluorescent protein

Template:ABSTRACT PUBMED 16369541

About this Structure

2B3Q is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Engineering and characterization of a superfolder green fluorescent protein., Pedelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS, Nat Biotechnol. 2006 Jan;24(1):79-88. Epub 2005 Dec 20. PMID:16369541

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